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• W1016012715 abstract "This chapter discusses the several aspects of the formation of shikimate, and with its conversion to aromatic amino acids. Although the role of D-glucose is stressed in the title, it should be pointed out that pyruvate and acetate, the starting materials for the other known pathways, may also be derived ultimately from D-glucose. A bacterial culture in a minimal medium (salts plus D-glucose) was irradiated with ultraviolet light, and the resultant mixture of wild-type and mutant strains was grown on a medium supplemented with yeast extract and casein hydrolyzate. Mutant strains were obtained which required for growth phenylalanine, tyrosine, tryptophan, and p-aminobenzoic acid. For certain of these strains, it was found that, of more than 50 compounds tested, only shikimic acid was able to replace the required aromatic supplement; other strains accumulated shikimic acid in the culture medium. It was therefore concluded that the former can convert shikimic acid to the aromatic metabolites, the genetic block in these organisms being somewhere before shikimic acid; the latter clearly were blocked after shikimic acid. Certain multiple aromatic auxotrophs grew slowly on the quadruple aromatic supplement, but were stimulated by the further addition of a trace of shikimic acid or of wild-type filtrate. The compound responsible for this effect was shown to be p-hydroxybenzoic acid, a bacterial vitamin subsequently found to be involved in the biosynthesis of methionine and lysine." @default.
• W1016012715 created "2016-06-24" @default.
• W1016012715 creator A5080198728 @default.
• W1016012715 date "1961-01-01" @default.
• W1016012715 modified "2023-10-14" @default.
• W1016012715 title "The Biosynthesis of Aromatic Compounds from D-Glucose" @default.
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