Matches in SemOpenAlex for { <https://semopenalex.org/work/W1016325037> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W1016325037 endingPage "88" @default.
- W1016325037 startingPage "675" @default.
- W1016325037 abstract "Endothelin-1 (ET-1) enhances the biosynthesis of interleukin-6 (IL-6) in endothelial cells and bone marrow-derived stromal cells of the rat. This study investigates (i) whether ET-1 stimulates the formation of tumour necrosis factor alpha (TNF alpha) or interferon-gamma (IFN gamma) in cultured macrophages or in the anaesthetized rat. Incubation of J774.2 macrophages with ET-1 (0.001-1 microM) caused a concentration- and time-dependent increase in the concentration of TNF alpha, but not of IFN gamma, in the culture medium. The increase in TNF alpha caused by stimulation of J774.2 macrophages was abolished by pretreatment of cells with (i) the protein synthesis inhibitor cycloheximide, (ii) with the selective ETA-receptor antagonists BQ-123 or BQ-485 (but not the selective ETB-receptor antagonist BQ-788), (iii) the tyrosine kinase inhibitors genistein or tyrphostin AG126, or (iv) with the glucocorticoid, dexamethasone. The inhibition by dexamethasone of the formation of TNF alpha by cells activated with ET-1 is not due to the formation of lipocortin-1 (LC1), as it was not reduced by a monoclonal antibody against LC1. Systemic administration (i.v.) of ET-1 (1 nmol.kg-1) to anaesthetized rats caused a rapid and sustained (maximum: 45 min; return to baseline: within 180 min) rise in the plasma levels of TNF alpha. This is the first demonstration that ET-1 can release proinflammatory cytokines in vitro and in vivo. The generation of TNF alpha caused by ET-1 involves (in sequence) the (i) activation of ETA-receptors, (ii) activation of tyrosine kinase resulting in the phosphorylation of intracellular proteins, (iii) the activation of, hitherto, unknown transcription factors, finally resulting in (iv) transcription and translation of the TNF alpha gene. The generation of TNF alpha by cells activated with ET-1 points to a pro-inflammatory role of ET-1 in diseases associated with local (e.g. atherosclerosis, heart failure) or systemic inflammation (circulatory shock), which are associated with high ET-1 plasma levels." @default.
- W1016325037 created "2016-06-24" @default.
- W1016325037 creator A5011307246 @default.
- W1016325037 creator A5015404078 @default.
- W1016325037 date "1997-12-01" @default.
- W1016325037 modified "2023-10-10" @default.
- W1016325037 title "Endothelin-1 stimulates the biosynthesis of tumour necrosis factor in macrophages: ET-receptors, signal transduction and inhibition by dexamethasone." @default.
- W1016325037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9444616" @default.
- W1016325037 hasPublicationYear "1997" @default.
- W1016325037 type Work @default.
- W1016325037 sameAs 1016325037 @default.
- W1016325037 citedByCount "51" @default.
- W1016325037 countsByYear W10163250372012 @default.
- W1016325037 countsByYear W10163250372013 @default.
- W1016325037 countsByYear W10163250372014 @default.
- W1016325037 countsByYear W10163250372016 @default.
- W1016325037 countsByYear W10163250372018 @default.
- W1016325037 countsByYear W10163250372019 @default.
- W1016325037 countsByYear W10163250372021 @default.
- W1016325037 countsByYear W10163250372022 @default.
- W1016325037 countsByYear W10163250372023 @default.
- W1016325037 crossrefType "journal-article" @default.
- W1016325037 hasAuthorship W1016325037A5011307246 @default.
- W1016325037 hasAuthorship W1016325037A5015404078 @default.
- W1016325037 hasConcept C126322002 @default.
- W1016325037 hasConcept C134018914 @default.
- W1016325037 hasConcept C149601957 @default.
- W1016325037 hasConcept C153911025 @default.
- W1016325037 hasConcept C164027704 @default.
- W1016325037 hasConcept C170493617 @default.
- W1016325037 hasConcept C17991360 @default.
- W1016325037 hasConcept C185592680 @default.
- W1016325037 hasConcept C2776914184 @default.
- W1016325037 hasConcept C2778128677 @default.
- W1016325037 hasConcept C2778690821 @default.
- W1016325037 hasConcept C3675279 @default.
- W1016325037 hasConcept C71924100 @default.
- W1016325037 hasConcept C86803240 @default.
- W1016325037 hasConceptScore W1016325037C126322002 @default.
- W1016325037 hasConceptScore W1016325037C134018914 @default.
- W1016325037 hasConceptScore W1016325037C149601957 @default.
- W1016325037 hasConceptScore W1016325037C153911025 @default.
- W1016325037 hasConceptScore W1016325037C164027704 @default.
- W1016325037 hasConceptScore W1016325037C170493617 @default.
- W1016325037 hasConceptScore W1016325037C17991360 @default.
- W1016325037 hasConceptScore W1016325037C185592680 @default.
- W1016325037 hasConceptScore W1016325037C2776914184 @default.
- W1016325037 hasConceptScore W1016325037C2778128677 @default.
- W1016325037 hasConceptScore W1016325037C2778690821 @default.
- W1016325037 hasConceptScore W1016325037C3675279 @default.
- W1016325037 hasConceptScore W1016325037C71924100 @default.
- W1016325037 hasConceptScore W1016325037C86803240 @default.
- W1016325037 hasIssue "4" @default.
- W1016325037 hasLocation W10163250371 @default.
- W1016325037 hasOpenAccess W1016325037 @default.
- W1016325037 hasPrimaryLocation W10163250371 @default.
- W1016325037 hasRelatedWork W1557263829 @default.
- W1016325037 hasRelatedWork W1977918131 @default.
- W1016325037 hasRelatedWork W2016872567 @default.
- W1016325037 hasRelatedWork W2021205826 @default.
- W1016325037 hasRelatedWork W2025333391 @default.
- W1016325037 hasRelatedWork W2054709772 @default.
- W1016325037 hasRelatedWork W2093278568 @default.
- W1016325037 hasRelatedWork W2106712361 @default.
- W1016325037 hasRelatedWork W2151614054 @default.
- W1016325037 hasRelatedWork W2253821600 @default.
- W1016325037 hasVolume "48" @default.
- W1016325037 isParatext "false" @default.
- W1016325037 isRetracted "false" @default.
- W1016325037 magId "1016325037" @default.
- W1016325037 workType "article" @default.