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- W1016503148 abstract "Some larval insects generate an extremely alkaline midgut segment (pH > 10). Previously, this phenomenon was studied with lepidopteran larvae where K+ is actively secreted by apical V-ATPases and K+/2H+ exchangers. Formally, this mechanism absorbs H+ and was therefore proposed as the source of alkalization ( Wieczorek, et al., 1991: J. Biol. Chem. 266, 15340). However, alkalization declines after mounting the isolated tissue, whereas K+ secretion stays high, suggesting that the two processes are independent ( Clark, et al., 1998: CBP 121A, 181). For the anterior stomach of larval mosquitoes, basolateral HCl absorption (V-ATPases, Cl− channels) and apical HCO3− secretion (Cl−/HCO3− exchangers) were proposed to essentially contribute to alkalization ( Boudko, et al., 2001: PNAS 98, 15354). In the present study, isolated stomachs of larval A. aegypti were mounted on perfusion pipettes and bathed in high buffer mosquito saline. With low buffer perfusion saline, containing m-Cresol Purple (mCP), transepithelial voltage was monitored and luminal alkalization became visible through colour changes of mCP after perfusion stop. Lumen negative voltage and alkalization depended on metabolic energy and were stimulated in the presence of serotonin (0.2 ìM). In some experiments a pH microelectrode in the lumen recorded pH values of up to 10 within minutes after perfusion stop. These results show that the isolated and perfused anterior stomach of larval A. aegypti is a promising preparation to analyze alkalization at the tissue level. First results with inhibitors and ion substitutions generate doubt about the contribution of the above-mentioned transporters. We are grateful for financial support by NSF (IBN-0091208)." @default.
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- W1016503148 title "Alkalization in the isolated anterior stomach of larval Aedes aegypti" @default.
- W1016503148 doi "https://doi.org/10.1096/fasebj.20.5.a1227-a" @default.
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