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- W1018202022 abstract "[Objective] The aim of the study was to make research on genomic structure variation and variety analysis of Dongxiang wild rice.[Method] Introgression groups of BC1F6 were based on donor of O.rufipogon Griff.and receptor of O.sativa sp.indica Kato..Strains of 239 in the group were analyzed on Polymorphism with the help of 25 couples of SSR primers distributed in 12 pairs of chromosomes.[Result] Gene fragments of O.rufipogon Griff.were found penetrated in the 25 microsatellite sites and most of the groups kept the parents of Xieqinzao B or DNA sequence of O.rufipogon Griff..The average rate of recurrent homozygous bands was 78.13% in the ILs,but the highest was 94.98%(amplified by primer RM131) and the lowest was 60.25%(RM171).The average rate of donor homozygous bands was 13.37%,but the highest was 32.64%(RM171) and the lowest was 2.93%(RM1095).There were numerous heterozygous sites in the population and the average heterozygosis rate was 5.62%,while the highest was 10.04%(RM401).Moreover,we found that there were some parental fragments lost and some novel fragments which were not detected in either parent in BC1F6 population.The average rate of lost bands was 2.88%,while the highest was 13.39%(RM311) and the lowest was 0(RM401).The average rate of new bands was 1%.The average of Nei's gene diversity(He) and Shannon's Information index(I) were 0.276 and 0.457 respectively in high generation of introgression lines.[Conclusion] The study demonstrated that distant hybridization led to extensive genetic and epigenetic variations in high generation of introgression lines,which expanded the base of genetic variation and laid an important foundation for rice improvement and germplasm innovation." @default.
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- W1018202022 date "2012-01-01" @default.
- W1018202022 modified "2023-09-27" @default.
- W1018202022 title "Analysis on genomic structure changes and diversity of introgression lines in Dongxiang wild rice (Oryza rufipogon Griff.)." @default.
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