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- W1020861376 abstract "In order to establish a simple, rapid, sensitive and specific assay for diagnosis of nasopharyngeal carcinoma (NPC), we prepard Epstein-Barr virus (EBV) DNA polymerase antigen by genetic engineering. DNA polymerase was expressed in BL21(DE3) using pRSET vector. The expressed product was characterized by SDS-PAGE and Western-blot. The IgG antibody angainst DNA polymerase of EBV in sera of NPC patients can be testified by expressed DNA polymerase through Western-blot and ELISA. The DNA polymerase is divided into the front 1/3 and the last 2/3 A2 parts, on A2 the antigenicity of DNA ploymerase locates. The sensitivity and specificity of Western-blot for detecting A2/IgG antibody was 80% and 100% respectively. The ELISA test established to detect A2/IgG antibody in sera of 46 NPC patients and 46 non-NPC patients also showed high sensitivity (89%) and specificity (93%)." @default.
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- W1020861376 date "2000-01-01" @default.
- W1020861376 modified "2023-09-26" @default.
- W1020861376 title "Study on the Expression and Purification of Epstein-Barr Virus DNA Polymerase and Its Application to Diagnosis of Nasopharyngeal Carcinoma" @default.
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