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- W102164920 abstract "The directed differentiation of human embryonic stem cells (hESCs) along a desired lineage has generated much promise in the field of regenerative medicine. To determine whether such subpopulations indicate specific tissue fates or represent a stochastic distribution within proliferating hESC cultures, we used FACS to sort CD133-positive hESCs from proliferating cultures constitutively expressing GFP, and co-cultured these CD133+/GFP+ cells with unselected, GFP-negative hESCs. At various timepoints in culture under proliferating conditions, GFP+ hESCs were re-analyzed by flow cytometry for the persistence of CD133 expression as well as the presence of other ubiquitous and distinct surface antigens (Tra-1-60, SSEA-4, FGFR-1, CD135). This demonstrated that CD133+GFP+ hESCs continue to express CD133 over serial passage, and are enriched for SSEA-4 expression as well. Upon differentiation in vitro, CD133+GFP+ hESCs co-cultured with unselected, GFP-negative hESCs primarily gave rise to neuroectoderm, as detected by co-expression of GFP and nestin (determined by immunostaining) in a characteristic fibrillar pattern. Tissues representing endoderm (anti-alpha-fetoprotein) and mesoderm (anti-smooth muscle actin) were not seen among GFP+ tissues. These data establish that CD133 marks a subpopulation of proliferating hESCs whose tissue fate is determined, and brings new relevance to the existence of distinct surface markers found in “pluripotent” populations of proliferating hESCs. This selection and co-culture approach also will enable similar analysis of other distinct hESC subpopulations for their use in the study of tissue development and potential therapeutic applications. This work was supported by NHLBI and the California Institute for Regenerative Medicine." @default.
- W102164920 created "2016-06-24" @default.
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- W102164920 date "2008-03-01" @default.
- W102164920 modified "2023-10-17" @default.
- W102164920 title "CD133+ subpopulations of pluripotent human embryonic stem cells differentiate into neuroectoderm" @default.
- W102164920 doi "https://doi.org/10.1096/fasebj.22.1_supplement.576.1" @default.
- W102164920 hasPublicationYear "2008" @default.
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