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- W1021805659 abstract "Attempts at isolation and culture of mesophyll protoplasts of tree species has revealed problems of low yields (1,2) coupled with poor digestibility which has necessitated lengthy exposure times to enzymes. There have also been problems of leaf leachates (3). Only recently, a case of successful mesophyll protoplast culture has been reported (4). Some researchers have overcome these problems by isolating protoplasts from callus cultures derived from mature trees (5,6) and have reported the complete protoplast systems utilizing such tissues for Santalum and Ulmus respectively. It has now been possible to induce embryogenic cell suspension of Populus ciliata. The somatic embryos produced by this cell suspension developed into complete plants which have been transferred to field conditions (7,8). This study was undertaken to determine the suitability of totipotent cell suspensions for isolation and culture of protoplasts." @default.
- W1021805659 created "2016-06-24" @default.
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- W1021805659 date "1988-01-01" @default.
- W1021805659 modified "2023-09-28" @default.
- W1021805659 title "Isolation and Culture of Protoplasts from Totipotent Cell Cultures of Populus Ciliata" @default.
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- W1021805659 doi "https://doi.org/10.1007/978-94-009-2788-9_37" @default.
- W1021805659 hasPublicationYear "1988" @default.
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