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- W102189764 abstract "籽粒硬度是小麦品质改良的重要目标性状之一,主要由5D短臂上的Hardness（Ha）位点的两个主效基因,即Puroindoline a（Pina-D1）和Puroindoline b（Pinb-D1）控制,同时受基因Grain Softness Protein-1（Gsp-1）的影响。本研究以一粒小麦（T.monococcum）DV92作为Pina^m、Pinb^m和Gsp^m基因的供体,将三基因各自完整的表达盒串联连接到载体pGEM-TEasy上,构建Pina^m-Pinb^m-Gsp^m三基因串联的表达载体。利用基因枪介导的转化方法转化普通小麦科农199幼胚,共轰击5608个小麦幼胚愈伤,经Biolaphos（化学除草剂）筛选,获得933株再生植株,经PCR检测,鉴定出11株阳性植株,有关转基因小麦的籽粒硬度还需进一步实验验证;本实验实现了串联三基因在普通小麦中的遗传转化,为利用基因枪介导的遗传转化方法改善小麦籽粒硬度提供了可行性。" @default.
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- W102189764 date "2012-01-01" @default.
- W102189764 modified "2023-09-23" @default.
- W102189764 title "Pina^m-Pinb^m-Gsp^m串联三基因表达载体的构建及其在普通小麦中的转化" @default.
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