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- W1022642962 abstract "Publisher Summary Genetic techniques have significantly contributed to the elucidation of metabolic pathways in microorganisms and to the knowledge of gene regulation. This chapter presents basic bacterial genetic techniques that can be easily employed in almost any biochemistry laboratory without extensive additional equipment or preparation. Salmonella typhimurium or Escherichia coli have been extensively investigated both genetically and biochemically. The body of information and the large number of mutant strains available make it extremely advantageous to use both of these organisms whenever possible in investigating new pathways. A wide variety of minimal and complete media are used in various laboratories. The minimal medium of Vogel and Bonner has the practical advantage that it can be made up as a 50-fold concentrate. This concentrate can be stored at room temperature and diluted when needed. A considerable amount of media preparation time is thus saved. Some of the powerful mutagens used for the induction of genetic changes include diethyl sulfate (DES), 1-Methyl-3-nitro-1-nitrosoguanidine (MNG), and ICR191. Various types of genetic techniques are used for the classification of mutations—namely, transductional methods, F' episomes, cross feeding, alternate-growth supplements, and direct-enzyme assays." @default.
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- W1022642962 date "1970-01-01" @default.
- W1022642962 modified "2023-09-27" @default.
- W1022642962 title "[1] Genetic techniques in studies of bacterial metabolism" @default.
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- W1022642962 doi "https://doi.org/10.1016/0076-6879(71)17165-0" @default.
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