Matches in SemOpenAlex for { <https://semopenalex.org/work/W1023183953> ?p ?o ?g. }
- W1023183953 endingPage "39" @default.
- W1023183953 startingPage "30" @default.
- W1023183953 abstract "Maklamicin, which is produced by the endophytic Micromonospora sp. NBRC 110955, is a spirotetronate-class antibiotic possessing anti-microbial activity against Gram-positive bacteria, and has several unique structural features different from other spirotetronates. Here we describe identification and characterization of the maklamicin biosynthetic (mak) gene cluster through draft genome sequencing, genomic library screening, and gene disruption. Sequence analysis revealed that a plausible maklamicin cluster resides in a 152 kb DNA region encoding 46 open reading frames, 24 of which can be assigned roles in the biosynthesis of polyketide backbone, spirotetronate or peripheral moieties, self-resistance and the regulation of maklamicin production. Disruption of the polyketide synthase (PKS) genes makA1 or makA4 resulted in a complete loss of maklamicin production, indicating that the type I modular PKS system is responsible for the biosynthesis of maklamicin. The mak gene cluster contained a set of biosynthetic genes for the formation of a tetronate moiety, which were found to be highly conserved in the gene clusters for spirotetronate antibiotics. Based on the estimated biosynthetic genes, we propose the biosynthetic pathway for maklamicin. Our findings provide not only insights on the biosynthetic mechanism of the unique structures in maklamicin, but also useful information to facilitate a comparative analysis of the spirotetronate biosynthetic pathways to expand the structural repertoire." @default.
- W1023183953 created "2016-06-24" @default.
- W1023183953 creator A5002257038 @default.
- W1023183953 creator A5030001281 @default.
- W1023183953 creator A5030394329 @default.
- W1023183953 creator A5051175802 @default.
- W1023183953 creator A5055131198 @default.
- W1023183953 creator A5071994729 @default.
- W1023183953 creator A5084261292 @default.
- W1023183953 creator A5085826127 @default.
- W1023183953 date "2015-11-01" @default.
- W1023183953 modified "2023-09-30" @default.
- W1023183953 title "Characterization of the biosynthetic gene cluster for maklamicin, a spirotetronate-class antibiotic of the endophytic Micromonospora sp. NBRC 110955" @default.
- W1023183953 cites W1496011245 @default.
- W1023183953 cites W1980747143 @default.
- W1023183953 cites W1980769545 @default.
- W1023183953 cites W1981470600 @default.
- W1023183953 cites W1988948560 @default.
- W1023183953 cites W1989259240 @default.
- W1023183953 cites W1993006529 @default.
- W1023183953 cites W1994767237 @default.
- W1023183953 cites W1996390229 @default.
- W1023183953 cites W1998828951 @default.
- W1023183953 cites W2001924031 @default.
- W1023183953 cites W2008659388 @default.
- W1023183953 cites W2009125082 @default.
- W1023183953 cites W2012641635 @default.
- W1023183953 cites W2014888781 @default.
- W1023183953 cites W2023950913 @default.
- W1023183953 cites W2034011943 @default.
- W1023183953 cites W2050260681 @default.
- W1023183953 cites W2066645642 @default.
- W1023183953 cites W2069383905 @default.
- W1023183953 cites W2070208209 @default.
- W1023183953 cites W2077911023 @default.
- W1023183953 cites W2083264894 @default.
- W1023183953 cites W2084250420 @default.
- W1023183953 cites W2084447564 @default.
- W1023183953 cites W2086242455 @default.
- W1023183953 cites W2086416477 @default.
- W1023183953 cites W2090367881 @default.
- W1023183953 cites W2091008703 @default.
- W1023183953 cites W2095063801 @default.
- W1023183953 cites W2097118224 @default.
- W1023183953 cites W2100380533 @default.
- W1023183953 cites W2106192857 @default.
- W1023183953 cites W2107093868 @default.
- W1023183953 cites W2109687548 @default.
- W1023183953 cites W2121131775 @default.
- W1023183953 cites W2131840797 @default.
- W1023183953 cites W2142785321 @default.
- W1023183953 cites W2154170463 @default.
- W1023183953 cites W2171535756 @default.
- W1023183953 cites W2171555414 @default.
- W1023183953 cites W2952806656 @default.
- W1023183953 doi "https://doi.org/10.1016/j.micres.2015.07.003" @default.
- W1023183953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26505309" @default.
- W1023183953 hasPublicationYear "2015" @default.
- W1023183953 type Work @default.
- W1023183953 sameAs 1023183953 @default.
- W1023183953 citedByCount "11" @default.
- W1023183953 countsByYear W10231839532016 @default.
- W1023183953 countsByYear W10231839532017 @default.
- W1023183953 countsByYear W10231839532018 @default.
- W1023183953 countsByYear W10231839532019 @default.
- W1023183953 countsByYear W10231839532020 @default.
- W1023183953 countsByYear W10231839532023 @default.
- W1023183953 crossrefType "journal-article" @default.
- W1023183953 hasAuthorship W1023183953A5002257038 @default.
- W1023183953 hasAuthorship W1023183953A5030001281 @default.
- W1023183953 hasAuthorship W1023183953A5030394329 @default.
- W1023183953 hasAuthorship W1023183953A5051175802 @default.
- W1023183953 hasAuthorship W1023183953A5055131198 @default.
- W1023183953 hasAuthorship W1023183953A5071994729 @default.
- W1023183953 hasAuthorship W1023183953A5084261292 @default.
- W1023183953 hasAuthorship W1023183953A5085826127 @default.
- W1023183953 hasBestOaLocation W10231839531 @default.
- W1023183953 hasConcept C104317684 @default.
- W1023183953 hasConcept C141231307 @default.
- W1023183953 hasConcept C2777763344 @default.
- W1023183953 hasConcept C2778635478 @default.
- W1023183953 hasConcept C2779920670 @default.
- W1023183953 hasConcept C2781382891 @default.
- W1023183953 hasConcept C36857842 @default.
- W1023183953 hasConcept C523546767 @default.
- W1023183953 hasConcept C54355233 @default.
- W1023183953 hasConcept C553450214 @default.
- W1023183953 hasConcept C70721500 @default.
- W1023183953 hasConcept C86803240 @default.
- W1023183953 hasConceptScore W1023183953C104317684 @default.
- W1023183953 hasConceptScore W1023183953C141231307 @default.
- W1023183953 hasConceptScore W1023183953C2777763344 @default.
- W1023183953 hasConceptScore W1023183953C2778635478 @default.
- W1023183953 hasConceptScore W1023183953C2779920670 @default.
- W1023183953 hasConceptScore W1023183953C2781382891 @default.
- W1023183953 hasConceptScore W1023183953C36857842 @default.
- W1023183953 hasConceptScore W1023183953C523546767 @default.
- W1023183953 hasConceptScore W1023183953C54355233 @default.