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- W1023657295 abstract "The application of ion pre-activation with 266 nm ultraviolet (UV) laser irradiation combined with electron capture dissociation (ECD) is demonstrated to enhance top-down mass spectrometry sequence coverage of disulfide bond containing proteins. UV-based activation can homolytically cleave a disulfide bond to yield two separated thiol radicals. Activated ECD experiments of insulin and ribonuclease A containing three and four disulfide bonds, respectively, were performed. UV-activation in combination with ECD allowed the three disulfide bonds of insulin to be cleaved and the overall sequence coverage to be increased. For the larger sized ribonuclease A with four disulfide bonds, irradiation from an infrared laser (10.6 µm) to disrupt non-covalent interactions was combined with UV-activation to facilitate the cleavage of up to three disulfide bonds. Preferences for disulfide bond cleavage are dependent on protein structure and sequence. Disulfide bonds can reform if the generated radicals remain in close proximity. By varying the time delay between the UV-activation and the ECD events, it was determined that disulfide bonds reform within 10-100 msec after their UV-homolytic cleavage." @default.
- W1023657295 created "2016-06-24" @default.
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- W1023657295 date "2015-11-01" @default.
- W1023657295 modified "2023-10-17" @default.
- W1023657295 title "Enhancing protein disulfide bond cleavage by UV excitation and electron capture dissociation for top-down mass spectrometry" @default.
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- W1023657295 doi "https://doi.org/10.1016/j.ijms.2015.07.008" @default.
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