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- W102422182 abstract "There are many gene products of biological interest that cannot be studied simply because they cannot be obtained in quantities sufficient for biochemical analysis. Recombinant technology provides some new approaches to the solution of this problem. This chapter describes the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in only minute amounts in a phage-infected bacterial cell. The chapter describes the subsequent development of this plasmid into a vector system that offers the potential for efficiently expressing essentially any gene product in Escherichia coli. The cII protein of bacteriophage λ is a transcriptional activator that positively regulates expression from two different phage promoters. These two promoters are coordinately controlled by this effector, and this control is essential for normal integration of the phage into the host genome, that is, formation of a bacterial lysogen." @default.
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- W102422182 date "1983-01-01" @default.
- W102422182 modified "2023-09-25" @default.
- W102422182 title "Use of Phage λ Regulatory Signals to Obtain Efficient Expression of Genes in Escherichia coli" @default.
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- W102422182 doi "https://doi.org/10.1016/b978-0-12-372380-2.50005-6" @default.
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