Matches in SemOpenAlex for { <https://semopenalex.org/work/W1024331682> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W1024331682 endingPage "130" @default.
- W1024331682 startingPage "125" @default.
- W1024331682 abstract "The chapter presents a study on bioassay of paf-acether by rabbit platelet aggregation. In the case of paf-acether (PAF), the method of choice is a bioassay, because the immunoassays that are now being developed have not yet proved their usefulness in day-to-day use in the research laboratory. Physicochemical methods are hampered by the very low paf concentration typically encountered. The present biological method for quantitation of paf is based on the monitoring of platelet activation either assessing platelet aggregation in an aggregometer or monitoring the release of radiolabeled serotonin previously incorporated into platelets. The following criteria are used to distinguish paf from other platelet agonists: (1) Conditions of platelet activation, (2) normal and reversed phase chromatographic elution behavior identical to that of synthetic paf, (3) inactivation by phospholipases: A2, C, and D, and resistance to lipase from Rhizopus arrhizus, and (4) when enough of the mediator can be recovered, gas chromatography and mass spectrometry characteristics. The chapter describes the other methods, which are less frequently used, for example, the platelet-desensitizing activity of paf or, for some cellular species, the incorporation of [3H]acetate into paf. The platelet aggregation method is a very sensitive tool that can be used to detect as little as picogram quantities of paf." @default.
- W1024331682 created "2016-06-24" @default.
- W1024331682 creator A5011801004 @default.
- W1024331682 creator A5080194719 @default.
- W1024331682 creator A5088685952 @default.
- W1024331682 creator A5090474227 @default.
- W1024331682 date "1990-01-01" @default.
- W1024331682 modified "2023-10-13" @default.
- W1024331682 title "[14] Bioassay of paf-acether by rabbit platelet aggregation" @default.
- W1024331682 cites W120153834 @default.
- W1024331682 cites W1696734442 @default.
- W1024331682 cites W1696765043 @default.
- W1024331682 cites W1893528652 @default.
- W1024331682 cites W2004088054 @default.
- W1024331682 cites W2023615833 @default.
- W1024331682 cites W2045665609 @default.
- W1024331682 cites W2047166809 @default.
- W1024331682 cites W2060507826 @default.
- W1024331682 cites W2069495150 @default.
- W1024331682 cites W2134674910 @default.
- W1024331682 cites W2152311269 @default.
- W1024331682 doi "https://doi.org/10.1016/0076-6879(90)87016-v" @default.
- W1024331682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2233336" @default.
- W1024331682 hasPublicationYear "1990" @default.
- W1024331682 type Work @default.
- W1024331682 sameAs 1024331682 @default.
- W1024331682 citedByCount "37" @default.
- W1024331682 countsByYear W10243316822018 @default.
- W1024331682 countsByYear W10243316822019 @default.
- W1024331682 crossrefType "book-chapter" @default.
- W1024331682 hasAuthorship W1024331682A5011801004 @default.
- W1024331682 hasAuthorship W1024331682A5080194719 @default.
- W1024331682 hasAuthorship W1024331682A5088685952 @default.
- W1024331682 hasAuthorship W1024331682A5090474227 @default.
- W1024331682 hasConcept C104488531 @default.
- W1024331682 hasConcept C170493617 @default.
- W1024331682 hasConcept C185592680 @default.
- W1024331682 hasConcept C203014093 @default.
- W1024331682 hasConcept C2775864247 @default.
- W1024331682 hasConcept C2779020240 @default.
- W1024331682 hasConcept C2993802102 @default.
- W1024331682 hasConcept C3018697912 @default.
- W1024331682 hasConcept C43617362 @default.
- W1024331682 hasConcept C54355233 @default.
- W1024331682 hasConcept C55493867 @default.
- W1024331682 hasConcept C86803240 @default.
- W1024331682 hasConcept C89560881 @default.
- W1024331682 hasConceptScore W1024331682C104488531 @default.
- W1024331682 hasConceptScore W1024331682C170493617 @default.
- W1024331682 hasConceptScore W1024331682C185592680 @default.
- W1024331682 hasConceptScore W1024331682C203014093 @default.
- W1024331682 hasConceptScore W1024331682C2775864247 @default.
- W1024331682 hasConceptScore W1024331682C2779020240 @default.
- W1024331682 hasConceptScore W1024331682C2993802102 @default.
- W1024331682 hasConceptScore W1024331682C3018697912 @default.
- W1024331682 hasConceptScore W1024331682C43617362 @default.
- W1024331682 hasConceptScore W1024331682C54355233 @default.
- W1024331682 hasConceptScore W1024331682C55493867 @default.
- W1024331682 hasConceptScore W1024331682C86803240 @default.
- W1024331682 hasConceptScore W1024331682C89560881 @default.
- W1024331682 hasLocation W10243316821 @default.
- W1024331682 hasLocation W10243316822 @default.
- W1024331682 hasOpenAccess W1024331682 @default.
- W1024331682 hasPrimaryLocation W10243316821 @default.
- W1024331682 hasRelatedWork W1977939514 @default.
- W1024331682 hasRelatedWork W1981790872 @default.
- W1024331682 hasRelatedWork W1989013875 @default.
- W1024331682 hasRelatedWork W2052022851 @default.
- W1024331682 hasRelatedWork W2076348643 @default.
- W1024331682 hasRelatedWork W2092065725 @default.
- W1024331682 hasRelatedWork W2118103560 @default.
- W1024331682 hasRelatedWork W2417081495 @default.
- W1024331682 hasRelatedWork W2419060792 @default.
- W1024331682 hasRelatedWork W969476632 @default.
- W1024331682 isParatext "false" @default.
- W1024331682 isRetracted "false" @default.
- W1024331682 magId "1024331682" @default.
- W1024331682 workType "book-chapter" @default.