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- W1024904814 abstract "A major consideration in creating and analysing hybrid plasmids is to adopt a strategy that will minimize the amount of work involved in achieving a particular goal. The plasmids can be naturally occurring or can be recombinants constructed in vitro from restriction endonuclease-cleaved fragments ligated into a suitable cloning vector. In the case of naturally occurring plasmids, the aim of the analysis may be to determine their size and restriction maps, to examine whether they carry particular sequences, and to localize on the plasmid genomes the regions of interest. This chapter describes the analysis of hybrid plasmids. The methods useful for the analysis of large and low copy number plasmids, which are more likely to occur among naturally occurring plasmids, are also discussed. The chapter outlines the various steps in clone analysis, reviewing recent developments and describing some of the standard methods that are in current use." @default.
- W1024904814 created "2016-06-24" @default.
- W1024904814 creator A5077540539 @default.
- W1024904814 date "1984-01-01" @default.
- W1024904814 modified "2023-09-27" @default.
- W1024904814 title "9 Analysis of Clones" @default.
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- W1024904814 doi "https://doi.org/10.1016/s0580-9517(09)70057-1" @default.
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