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- W1025402846 abstract "Publisher Summary This chapter describes electrophysiological techniques used for the demonstration of low-resistance junctions by the occurrence of ionic coupling pulses. For the determination of ionic coupling between nonexcitable cells, it is necessary to measure (1) the potential difference(PD) between the cell interior and the medium, (2) the superimposed pulses resulting from the injection of current pulses into a cell, and (3) the resistances of the cell membrane and junctional membrane (low-resistance junctions) to show the integrity of the cell. Most of the instruments used for pulse generation, amplification, and recording are commercially available. It is shown that for the analysis of coupling phenomena between cells, electrical parameters such as potential difference, membrane resistance, and capacitance must be taken into account. In addition, the electrical and mechanical instrumentation necessary for such measurements are described with special emphasis on electrophysiological studies in cultured cells. Based on experiments with lymphocytes it is shown that low-resistance junctions can be built up within minutes and that in the case of cells of established lines in culture, their existence is related neither to normal nor to malignant cell properties, but appears to be associated with fibroblastoid cells." @default.
- W1025402846 created "2016-06-24" @default.
- W1025402846 creator A5019907279 @default.
- W1025402846 date "1974-01-01" @default.
- W1025402846 modified "2023-09-23" @default.
- W1025402846 title "Chapter 17 Ionic Coupling between Nonexcitable Cells in Culture" @default.
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- W1025402846 doi "https://doi.org/10.1016/s0091-679x(08)60456-2" @default.
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