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- W1027211134 abstract "This chapter focuses on preparation of prenyltransferases of pumpkin fruit. It discusses assay methods in which principle and procedure are described. The assay method determines the amount of 14C labeled isopentenyl pyrophosphate, which reacts with allylic substrates to form 14C-labeled compounds which, after acid treatment, are extractable with hexane. In the assay of crude preparations, it is recommended to use geranyl pyrophosphate plus [14C]isopentenyl pyrophosphate in the presence of Mn2+ and farnesyl pyrophosphate plus [14C]isopentenyl pyrophosphate in the presence of Mn2+for the assay of farnesylpyrophosphate synthetase and geranylgeranylpyrophosphate synthetase, respectively. The product of the farnesylpyrophosphate synthetase reaction starting with dimethylallyl pyrophosphate and isopentenyl pyrophosphate is a mixture of geranyl pyrophosphate and all-trans-farnesylpyrophosphate, whereas all-trans-geranylgeranyl pyrophosphate is the only product in the reaction catalyzed by geranylgeranylpyrophosphate synthetase regardless of the chain length of the allylicsubstrate with which the reaction is initiated. Purification of enzymes is also discussed in the chapter in which extraction and fractionation are described." @default.
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- W1027211134 title "[19] Prenyltransferases of pumpkin fruit" @default.
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- W1027211134 doi "https://doi.org/10.1016/s0076-6879(85)10072-8" @default.
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