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- W1027509776 abstract "Publisher Summary This chapter describes a method for mechanical blending of the tissue and purification of plastids on a self-generating isosmolar density gradient of Percoll. The method described produces plastids containing middle-size starch grains with a high degree of intactness. They approach ultimate purity as judged by electron microscopy and analysis using marker enzymes. Percoll-purified cauliflower bud plastids are thus suitable for a large range of studies. For example, the first exhaustive description of the enzymatic capacities of a nongreen plastid type for basic carbon metabolism (glycolysis, pentose-P pathway, and starch metabolism), nitrite assimilation, and lipid synthesis (long-chain fatty acid and galactolipid synthesis) can be drawn up by using purified cauliflower bud plastids. The maximal activities recorded for the enzymes involved in these pathways showed that cauliflower bud plastids are recovered in an excellent physiological state. Cauliflower bud plastids are also suitable for envelope phospholipid and pigment analysis, native plastid-DNA isolation, metabolite transport studies, and helps to increase the knowledge about these biochemically very flexible organelles." @default.
- W1027509776 created "2016-06-24" @default.
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- W1027509776 date "1987-01-01" @default.
- W1027509776 modified "2023-09-27" @default.
- W1027509776 title "[23] Isolation of plastids from buds of cauliflower (Brassica oleracea L.)" @default.
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- W1027509776 doi "https://doi.org/10.1016/0076-6879(87)48025-7" @default.
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