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- W1028206543 abstract "Publisher Summary This chapter discusses the determination of D-aldohexopyranoside dehydrogenase. The assay is based on the reduction of 2,6-dichlorophenolindophenol to its leuco form, coupled to the oxidation of methyl-α-D-glucopyranoside. One unit of enzyme catalyzes a decrease of 1 absorbance unit per minute at 28°. Specific activity is expressed as units per milligram protein. In the course of purification procedure, Agrobacterium tumefaciens strain B-6 (virulent) or II BNV-6 (avirulent) is maintained on potato dextrose agar slants containing 0.5% yeast extract. The cells are harvested by centrifugation, washed twice by suspension in H2O and centrifugation, and then resuspended in H2O. Aerated resting cell preparations (in buffer) can be used to prepare a number of glycosid-3-uloses from D-glucosides and D-galactosides. Crude extract and purified fractions up to and including the heat-step supernatant are stable for at least 48 hours at 0–4°. More highly purified enzyme is less stable and loses about 50% of its activity over a 24-hour period." @default.
- W1028206543 created "2016-06-24" @default.
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- W1028206543 date "1966-01-01" @default.
- W1028206543 modified "2023-09-27" @default.
- W1028206543 title "[17] d-aldohexopyranoside dehydrogenase" @default.
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- W1028206543 doi "https://doi.org/10.1016/0076-6879(66)09021-9" @default.
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