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- W1029130855 abstract "Rap1, a Ras-like small GTPase, plays a crucial role in cell-matrix adhesive interactions, cell-cell junction formation, cell polarity and migration. The role of Rap1 in vertebrate organ development and tissue architecture, however, remains elusive. We addressed this question in a mouse lens model system using a conditional gene targeting approach. While individual germline deficiency of either Rap1a or Rap1b did not cause overt defects in mouse lens, conditional double deficiency (Rap1 cKO) prior to lens placode formation led to an ocular phenotype including microphthalmia and lens opacification in embryonic mice. The embryonic Rap1 cKO mouse lens exhibited striking defects including loss of E-cadherin- and ZO-1-based cell-cell junctions, disruption of paxillin and β1-integrin-based cell adhesive interactions along with abnormalities in cell shape and apical-basal polarity of epithelium. These epithelial changes were accompanied by increased levels of α-smooth muscle actin, vimentin and N-cadherin, and expression of transcriptional suppressors of E-cadherin (Snai1, Slug and Zeb2), and a mesenchymal metabolic protein (Dihydropyrimidine dehydrogenase). Additionally, while lens differentiation was not overtly affected, increased apoptosis and dysregulated cell cycle progression were noted in epithelium and fibers in Rap1 cKO mice. Collectively these observations uncover a requirement for Rap1 in maintenance of lens epithelial phenotype and morphogenesis." @default.
- W1029130855 created "2016-06-24" @default.
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- W1029130855 date "2015-10-01" @default.
- W1029130855 modified "2023-10-15" @default.
- W1029130855 title "Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis" @default.
- W1029130855 cites W12760659 @default.
- W1029130855 cites W140943306 @default.
- W1029130855 cites W1488252042 @default.
- W1029130855 cites W1493744668 @default.
- W1029130855 cites W1964081206 @default.
- W1029130855 cites W1970336112 @default.
- W1029130855 cites W1974791474 @default.
- W1029130855 cites W1979195041 @default.
- W1029130855 cites W1983790767 @default.
- W1029130855 cites W1984246470 @default.
- W1029130855 cites W1987234522 @default.
- W1029130855 cites W1991029044 @default.
- W1029130855 cites W1991356125 @default.
- W1029130855 cites W1995115677 @default.
- W1029130855 cites W2007180015 @default.
- W1029130855 cites W2011409902 @default.
- W1029130855 cites W2012551249 @default.
- W1029130855 cites W2013549218 @default.
- W1029130855 cites W2026851415 @default.
- W1029130855 cites W2027235566 @default.
- W1029130855 cites W2027577700 @default.
- W1029130855 cites W2032646886 @default.
- W1029130855 cites W2032942532 @default.
- W1029130855 cites W2037389941 @default.
- W1029130855 cites W2041954573 @default.
- W1029130855 cites W2042570069 @default.
- W1029130855 cites W2047580046 @default.
- W1029130855 cites W2050989809 @default.
- W1029130855 cites W2051267706 @default.
- W1029130855 cites W2052746404 @default.
- W1029130855 cites W2056811680 @default.
- W1029130855 cites W2059253566 @default.
- W1029130855 cites W2060828441 @default.
- W1029130855 cites W2065869497 @default.
- W1029130855 cites W2066378083 @default.
- W1029130855 cites W2067733585 @default.
- W1029130855 cites W2069878364 @default.
- W1029130855 cites W2071570050 @default.
- W1029130855 cites W2071596809 @default.
- W1029130855 cites W2073461890 @default.
- W1029130855 cites W2075964588 @default.
- W1029130855 cites W2080425802 @default.
- W1029130855 cites W2082125951 @default.
- W1029130855 cites W2095035711 @default.
- W1029130855 cites W2095397788 @default.
- W1029130855 cites W2096638905 @default.
- W1029130855 cites W2100338747 @default.
- W1029130855 cites W2101379456 @default.
- W1029130855 cites W2104310645 @default.
- W1029130855 cites W2104546896 @default.
- W1029130855 cites W2107601924 @default.
- W1029130855 cites W2108496308 @default.
- W1029130855 cites W2109619688 @default.
- W1029130855 cites W2111297349 @default.
- W1029130855 cites W2112718652 @default.
- W1029130855 cites W2117933964 @default.
- W1029130855 cites W2118263188 @default.
- W1029130855 cites W2118947728 @default.
- W1029130855 cites W2123179922 @default.
- W1029130855 cites W2128201178 @default.
- W1029130855 cites W2131142658 @default.
- W1029130855 cites W2132793070 @default.
- W1029130855 cites W2134321528 @default.
- W1029130855 cites W2135577930 @default.
- W1029130855 cites W2142820370 @default.
- W1029130855 cites W2144414716 @default.
- W1029130855 cites W2145009320 @default.
- W1029130855 cites W2146831137 @default.
- W1029130855 cites W2152059614 @default.
- W1029130855 cites W2153975342 @default.
- W1029130855 cites W2154589460 @default.
- W1029130855 cites W2156299846 @default.
- W1029130855 cites W2158678196 @default.
- W1029130855 cites W2161162896 @default.
- W1029130855 cites W2162507785 @default.
- W1029130855 cites W2163542262 @default.
- W1029130855 cites W2164358503 @default.
- W1029130855 cites W2172120237 @default.
- W1029130855 cites W2597632520 @default.
- W1029130855 cites W4210370525 @default.
- W1029130855 cites W4250731522 @default.
- W1029130855 doi "https://doi.org/10.1016/j.ydbio.2015.06.022" @default.
- W1029130855 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4587029" @default.
- W1029130855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26212757" @default.
- W1029130855 hasPublicationYear "2015" @default.
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