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- W1029261710 abstract "This chapter provides evidence for a transport of G protein in vitro between distinct compartments of the Golgi apparatus. Transport is dependent on the presence of adenosine triphosphate (ATP) and the addition of a cytosol fraction. Two methods for measuring transport-coupled oligosaccharide procesing in vitro are discussed in this chapter. In the first case, the polypeptide backbone of G protein is labeled and oligosaccharide processing is inferred from Endo H resistance, whereas the second method follows glycosylation of G directly via incorporation of the [3H]GIcNAc into unlabeled G proteins. The assay based on the conferral of resistance to Endo H provides information regarding the location and nature of the donor but requires a minimum of 2–3 days postincubation processing to complete the analysis. The assay based on the incorporation of [3H]GIcNAc into G protein from its sugar-nucleotide precursor is simpler, rapid, and quantitative, making it the assay of choice to be employed for purification and characterization of components required for G protein transport." @default.
- W1029261710 created "2016-06-24" @default.
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- W1029261710 date "1983-01-01" @default.
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- W1029261710 title "[4] Transport-coupled oligosaccharide processing in a cell-free system" @default.
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- W1029261710 doi "https://doi.org/10.1016/0076-6879(83)98137-5" @default.
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