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- W1030147982 abstract "Publisher Summary The excellent resolution and simplicity of analytical gel electrophoresis led to attempts to scale up the analytical procedure and to attain a high resolution of the components of macromolecular species and their isolation by gel electrophoresis on a preparative scale. The gel mixture is polymerized for one hour at the temperature at which the electrophoretic separation is to be performed. Joule heat produced in electrophoretic experiments does not permit a thickness of the acrylamide gel layer of greater than 15 mm to be used, otherwise the heat cannot be evenly dissipated and curvature of migrating bands occurs. The electrophoresis time is inversely proportional to the voltage gradient and therefore the use of high voltage gradients would be preferable. However, the Joule heat limits the maximal voltage to ca. 10 V/cm in the separation gel. The temperature of gel also influences the resolution, as with the increasing temperature the electrophoretic mobility of the macro-ions increases, with resulting shortening of the electrophoretic time and a decrease in zone spreading." @default.
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- W1030147982 date "1979-01-01" @default.
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- W1030147982 title "Chapter 13 Preparative Electrophoresis in Gel Media" @default.
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- W1030147982 doi "https://doi.org/10.1016/s0301-4770(08)60889-1" @default.
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