Matches in SemOpenAlex for { <https://semopenalex.org/work/W1032652208> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1032652208 endingPage "629" @default.
- W1032652208 startingPage "612" @default.
- W1032652208 abstract "The chapter discusses freeze-drying and high-resolution shadowing in electron microscopy of Escherichia coli ribosomes. Freezing makes it possible to fix the preparation instantly without any change of the initial conditions. Thus, it may be used to investigate the labile structures as well as the kinetics of some conformational transitions. Freeze-drying in vacuum preserves the object from deformation which arises at air-drying, apparently owing to the action of capillary forces. It has been shown, for example, that ribosomal particles freeze-dried in vacuum have dimensions 20-25% greater than the air-dried ribosomes. At freeze-drying in vacuum, redistribution does not occur. This is one of the reasons why electron micrographs of the object freeze-dried in vacuum are more informative than the micrographs of the same object air-dried. The requirements for the quality of shadowing are higher in freeze-drying. It is preferable to use metals that melt at high temperatures. They give high-contrast thin layers with a very small granularity, which are not crystallized under the electron beam. This permits shadowing at steeper angles relative to the specimen plane, gives a minimum of distortions due to the metal cap, and leads to a better revealing of the shape and the surface relief of the object. Application of the method for investigation of the small ribosomal subparticle of E. coli permitted the study not only of its morphology, but also of its internal organization." @default.
- W1032652208 created "2016-06-24" @default.
- W1032652208 creator A5036827827 @default.
- W1032652208 creator A5040674884 @default.
- W1032652208 date "1979-01-01" @default.
- W1032652208 modified "2023-10-18" @default.
- W1032652208 title "[48] Freeze-drying and high-resolution shadowing in electron microscopy of Escherichia coli ribosomes" @default.
- W1032652208 cites W1564216598 @default.
- W1032652208 cites W1991108535 @default.
- W1032652208 cites W2009223706 @default.
- W1032652208 cites W2014860689 @default.
- W1032652208 cites W2046293760 @default.
- W1032652208 cites W2071010415 @default.
- W1032652208 cites W2078204240 @default.
- W1032652208 cites W2084124582 @default.
- W1032652208 cites W2089848658 @default.
- W1032652208 cites W2139719143 @default.
- W1032652208 cites W2145534266 @default.
- W1032652208 doi "https://doi.org/10.1016/0076-6879(79)59117-4" @default.
- W1032652208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/374956" @default.
- W1032652208 hasPublicationYear "1979" @default.
- W1032652208 type Work @default.
- W1032652208 sameAs 1032652208 @default.
- W1032652208 citedByCount "22" @default.
- W1032652208 countsByYear W10326522082012 @default.
- W1032652208 countsByYear W10326522082013 @default.
- W1032652208 countsByYear W10326522082017 @default.
- W1032652208 crossrefType "book-chapter" @default.
- W1032652208 hasAuthorship W1032652208A5036827827 @default.
- W1032652208 hasAuthorship W1032652208A5040674884 @default.
- W1032652208 hasConcept C104317684 @default.
- W1032652208 hasConcept C111919701 @default.
- W1032652208 hasConcept C120665830 @default.
- W1032652208 hasConcept C121332964 @default.
- W1032652208 hasConcept C159467904 @default.
- W1032652208 hasConcept C177774035 @default.
- W1032652208 hasConcept C185592680 @default.
- W1032652208 hasConcept C192562407 @default.
- W1032652208 hasConcept C3018767501 @default.
- W1032652208 hasConcept C41008148 @default.
- W1032652208 hasConcept C43617362 @default.
- W1032652208 hasConcept C55493867 @default.
- W1032652208 hasConcept C67705224 @default.
- W1032652208 hasConcept C8010536 @default.
- W1032652208 hasConcept C84199377 @default.
- W1032652208 hasConcept C88478588 @default.
- W1032652208 hasConcept C93877712 @default.
- W1032652208 hasConceptScore W1032652208C104317684 @default.
- W1032652208 hasConceptScore W1032652208C111919701 @default.
- W1032652208 hasConceptScore W1032652208C120665830 @default.
- W1032652208 hasConceptScore W1032652208C121332964 @default.
- W1032652208 hasConceptScore W1032652208C159467904 @default.
- W1032652208 hasConceptScore W1032652208C177774035 @default.
- W1032652208 hasConceptScore W1032652208C185592680 @default.
- W1032652208 hasConceptScore W1032652208C192562407 @default.
- W1032652208 hasConceptScore W1032652208C3018767501 @default.
- W1032652208 hasConceptScore W1032652208C41008148 @default.
- W1032652208 hasConceptScore W1032652208C43617362 @default.
- W1032652208 hasConceptScore W1032652208C55493867 @default.
- W1032652208 hasConceptScore W1032652208C67705224 @default.
- W1032652208 hasConceptScore W1032652208C8010536 @default.
- W1032652208 hasConceptScore W1032652208C84199377 @default.
- W1032652208 hasConceptScore W1032652208C88478588 @default.
- W1032652208 hasConceptScore W1032652208C93877712 @default.
- W1032652208 hasLocation W10326522081 @default.
- W1032652208 hasLocation W10326522082 @default.
- W1032652208 hasOpenAccess W1032652208 @default.
- W1032652208 hasPrimaryLocation W10326522081 @default.
- W1032652208 hasRelatedWork W1032652208 @default.
- W1032652208 hasRelatedWork W2003662333 @default.
- W1032652208 hasRelatedWork W2004159340 @default.
- W1032652208 hasRelatedWork W2011234061 @default.
- W1032652208 hasRelatedWork W2071270428 @default.
- W1032652208 hasRelatedWork W2077159697 @default.
- W1032652208 hasRelatedWork W2094071787 @default.
- W1032652208 hasRelatedWork W2095025042 @default.
- W1032652208 hasRelatedWork W2057645468 @default.
- W1032652208 hasRelatedWork W2528356406 @default.
- W1032652208 isParatext "false" @default.
- W1032652208 isRetracted "false" @default.
- W1032652208 magId "1032652208" @default.
- W1032652208 workType "book-chapter" @default.