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- W1032652424 abstract "Publisher Summary The chemistry of the enzymatic reactions assayed by ion-exchange paper technique is described. This technique constitutes an example of the conversion of column ion-exchange technique to ascending paper chromatographic technique-utilizing paper loaded with an anion-exchange resin. All purine and pyrimidine precursors (with the exception of orotic acid) are weakly bound to the strongly basic ion-exchange resin, while the phosphorylated products of the nucleotide pyrophosphorylase reaction are more strongly bound. With the ready availability of radioactive purine and pyrimidine precursors, assay for the nucleotide pyrophosphorylase in extracts from very small amounts of tissue or in partially purified enzyme preparations can be achieved. The sensitivity depends on the specific activity of the available substrates. This assay procedure can be adapted to any of the assays except that use labeled orotic acid, because of the strong affinity of orotic acid for the resin. It can also be adapted to other assays where any weakly bound precursor is converted into a strongly bound product, for example, the conversion of thymidine to thymidylic acid." @default.
- W1032652424 created "2016-06-24" @default.
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- W1032652424 date "1967-01-01" @default.
- W1032652424 modified "2023-09-27" @default.
- W1032652424 title "[41] Ion-exchange paper techniques for assay of nucleotide pyrophosphorylase" @default.
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- W1032652424 doi "https://doi.org/10.1016/s0076-6879(67)12050-8" @default.
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