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- W1033677669 abstract "This chapter describes the purification to homogeneity of functional Sec23 protein using the endoplasmic reticulum (ER) -to- Golgi transport assay to monitor its activity. Temperature-sensitive mutations in SEC and BET genes are used to genetically define many of the proteins required for transport from the endoplasmic reticulum (ER) to the Golgi in yeast. The SEC23 gene product is an 84K protein that is peripherally associated with intracellular membrane and can be converted into a soluble form by a number of treatments, including vigorous mechanical agitation during yeast cell lysis. Sec23p appears to function in ER-to-Golgi transport during budding of a vesicular intermediate as transport vesicles do not form in sec23 yeast in vivo or in vitro. The assay has been used to purify active Sec23p and an associated 105K protein from cells carrying a single chromosomal copy of SEC23 and from cells overproducing Sec23p from multiple plasmid-borne copies of the gene." @default.
- W1033677669 created "2016-06-24" @default.
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- W1033677669 date "1992-01-01" @default.
- W1033677669 modified "2023-10-16" @default.
- W1033677669 title "[32] Purification of yeast sec23 protein by complementation of mutant cell lysates deficient in endoplasmic reticulum-to-Golgi transport" @default.
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- W1033677669 doi "https://doi.org/10.1016/0076-6879(92)19034-4" @default.
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