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- W1034255135 abstract "This chapter describes the assay procedure for the vitamin K1 2,3-epoxide-reducing system. The availability of an assay for the vitamin K1 2,3-epoxide-reducing system is important for studying the site of action of warfarin, and identifying compounds that inhibit the “resistant” enzyme and therefore have potential commercial importance as rodenticides. This chapter discusses an assay system in which high-performance liquid chromatography (HPLC) is used to separate and quantify the reaction product. The method used is a modification of that described by Matschiner. The vitamin K1 is purchased from Sigma (London) Ltd., and the 2,3-epoxide prepared from it by the method of Tishler is chromatographically pure. Male rats (190–210 g) from a Wistar-derived colony are killed by cervical dislocation and bled by cutting the blood vessels of the neck. The liver is removed and washed in ice-cold 0.9% saline and weighed. It is macerated in 0.25 M sucrose–25 mM potassium phosphate buffer, pH 7.4–5 mM dithiothreitol with a Potter-Elvehjem homogenizer fitted with a Teflon pestle and surrounded by iced water. For rat postmitochondrial supernatant, the assay was found to be linear over the range of 50–200 mg fresh weight of liver, and 5–40 min, the maximum activity occurring with 300 μM vitamin K1 2,3-epoxide)." @default.
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- W1034255135 date "1980-01-01" @default.
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- W1034255135 title "[21] Assay procedure for the Vitamin K1 2,3-epoxide-reducing system" @default.
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- W1034255135 doi "https://doi.org/10.1016/s0076-6879(80)67023-2" @default.
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