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- W1034338429 abstract "Publisher Summary This chapter describes a method for introduction of exogenous macromolecules into the cytoplasm of living D. discoideum amoebae. The most general purpose of the method is to extend molecular studies of cellular constituents from the test tube to the living cell, and to provide a link between the disciplines of cell biology and biochemistry. Thus, the biochemical characteristics of macromolecules predicted from their behavior in dilute solution in vitro can be examined intracellularly, and the functions of specific macrornolecules hypothesized from their structure and molecular interactions in vitro can be critically tested. The distributions of macromolecules in living cells may be observed, and the intracellular concentrations of potential secondary messenger molecules such as free calcium ions and protons that may modulate the structure and activity of cellular constituents may be examined. These measurements can be correlated both temporally and spatially with ongoing cellular activities including development and movement to elucidate the molecular mechanisms mediating and regulating such processes. A number of methods for introducing macromolecules into living cells are described. It is difficult to microinject D. discoideum , and only small numbers of cells can be microinjected in a short period of time." @default.
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- W1034338429 date "1987-01-01" @default.
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- W1034338429 title "Chapter 9 Introduction of Exogenous Molecules into the Cytoplasm of Dictyostelium discoideum Amoebae by Controlled Sonication" @default.
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- W1034338429 doi "https://doi.org/10.1016/s0091-679x(08)61644-1" @default.
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