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- W1034392564 abstract "将堆型艾美耳球虫保定株裂殖子3-1E基因,插入到真核表达载体pcDNA3.1（＋）中,构建重组表达质粒pcDNA-3-1E,用碱裂解法大量提取重组表达质粒,纯化后免疫接种1周龄雏鸡。分别设高（100μg）、中（50μg）、低（25μg）3个剂量组,同时设活卵囊接种组、非免疫感染组和健康对照组。在7日龄时首免,14日龄加强免疫,1周后用2.5×105个E.acervulina保定株球虫孢子化卵囊进行攻毒试验。结果显示50μg的剂量可使试验鸡产生较强的抗球虫效果,攻虫鸡的卵囊产量下降67.67%,肠道病变记分降低66.96%,并使鸡的相对增重率达88.36%,ACI值为176.06。" @default.
- W1034392564 created "2016-06-24" @default.
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- W1034392564 date "2011-01-01" @default.
- W1034392564 modified "2023-09-23" @default.
- W1034392564 title "堆型艾美耳球虫pcDNA3-3-1E重组表达质粒的免疫保护性" @default.
- W1034392564 hasPublicationYear "2011" @default.
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