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- W1034659848 abstract "Objective To observe the distribution of bacteria and morphologic features of biofilms(BFs) on the surface of endotracheal tubes(ETT) in PICU.Method 35 children undergoing mechanical ventilation(MV) were involved in this study.ETTs were collected at the first time of extubation.After staining with PI and FITC-ConA,the distribution of the bacteria and extracellular polymeric substances(EPS) were observed by confocal laser scanning microscopy(CLSM).Meanwhile,bacteria in BF and the secretions of lower respiratory tract were isolated,cultured and identified,respectively.Result(1)Of 35 ETTs,31 tubes were positive(88.57%).And 17 samples had the same pathogen both in the ETTs and secretions of lower respiratory tract,which accounted for 54.83%of the positive cultures from ETTs.Staphylococcus aureu,Klebsiella pneumoniae,Escherichia coli and Enterobacter cloacae were the main pathogens in BF and the secretions of lower respiratory tract.(2) Observation by CLSM showed that the bacteria were adhered on the surface of the ETTs in 12 hours after intubation;after 48 hours,lots of spherical or clubbed bacteria adhered into clusters,EPS also increased,and the initial BF was formed;After 72 hours,bacteria which encased in mass of EPS aggregated mutually into clumps,and formed mature BF;After more than 7 days the bacteria adhered into lamellars,and there were some microcolonies around mature BF.(3)Of 35 mechanical ventilated children,19 cases had VAP,including 10 in orotracheal intubation and 9 in nasotracheal intubation.Conclusion Bacterial biofilm can form on the surface of ETTs when ventilated.There may be correlation between microbial colonization and BF formation on the surface of ETTs and VAP in children who were intubated and ventilated for a long period." @default.
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- W1034659848 date "2009-01-01" @default.
- W1034659848 modified "2023-09-27" @default.
- W1034659848 title "The formation of bacterial biofilm and analysis of pathogens on tracheal tubes in ventilated children." @default.
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