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- W103494911 abstract "A Chinese Hamster Ovary (CHO) cell line containing the human gamma interferon (IFN-∝) gene co-amplified with the gene for dihydrofolate reductase (DHFR), was continuously maintained for 120 days in serum-free medium with 0.1μM methotrexate. During this time the IFN-∝ titre fell by 70% due to a reduction in the specific IFN production rate. Cell number was higher in late passage cultures and cell viability was maintained for longer periods. Chromosome studies revealed no significant difference in the overall chromosome frequency and distribution, except for a 16% increase in the modal chromosome number of 18. Southern blot analysis of DHFR showed a 45% decrease in levels over 120 days with IFN-∝ decreasing by 30% over the same time period. Keywords : CHO, stability, amplification, DHFR, interferon, methotrexate." @default.
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- W103494911 date "1991-01-01" @default.
- W103494911 modified "2023-10-16" @default.
- W103494911 title "STABILITY OF AMPLIFIED DNA IN CHINESE HAMSTER OVARY CELLS" @default.
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- W103494911 doi "https://doi.org/10.1016/b978-0-7506-1103-9.50063-9" @default.
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