Matches in SemOpenAlex for { <https://semopenalex.org/work/W1036150892> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1036150892 endingPage "653" @default.
- W1036150892 startingPage "641" @default.
- W1036150892 abstract "Publisher Summary This chapter describes the high-affinity interaction between antigen and surface immunoglobulin receptors on B cells in conjunction with the tight binding between avidin and biotin to achieve specific preselected cell–cell contact in suspensions to generate hybridoma. The procedures result in high-affinity monoclonal antibody production from hybrids formed during electro-fusion. The chemical conjugation of antigen to avidin is complex; variety of chemical cross-linking procedures may be employed. Conjugation to avidin is preferred over conjugation to biotin because of the relative ease of characterizing the avidinylated conjugate and the greater efficiency of biotinylating myeloma cells. Cross-linking reactions may be performed in solution or in the solid phase by immobilization of avidin onto iminobiotin–Sepharose. Ethylenediamine is combined with CNBr-activated Sepharose CL-4B and the product is allowed to react with the N -hydroxysuccinimide derivative of iminobiotin. The free (unreacted) amines of the iminobiotin–Sepharose preparation are blocked by reaction with Sanger's reagent." @default.
- W1036150892 created "2016-06-24" @default.
- W1036150892 creator A5043815688 @default.
- W1036150892 creator A5081119595 @default.
- W1036150892 date "1990-01-01" @default.
- W1036150892 modified "2023-09-24" @default.
- W1036150892 title "[76] Facilitated cell fusion for hybridoma production" @default.
- W1036150892 cites W1508861647 @default.
- W1036150892 cites W1516608985 @default.
- W1036150892 cites W2016188858 @default.
- W1036150892 cites W2016865253 @default.
- W1036150892 cites W2024504149 @default.
- W1036150892 cites W2028732607 @default.
- W1036150892 cites W2030522563 @default.
- W1036150892 cites W2032381660 @default.
- W1036150892 cites W2032949589 @default.
- W1036150892 cites W2061415244 @default.
- W1036150892 cites W2065861447 @default.
- W1036150892 cites W2075489180 @default.
- W1036150892 cites W2080130205 @default.
- W1036150892 cites W2146972602 @default.
- W1036150892 cites W4211027922 @default.
- W1036150892 doi "https://doi.org/10.1016/0076-6879(90)84330-j" @default.
- W1036150892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2167431" @default.
- W1036150892 hasPublicationYear "1990" @default.
- W1036150892 type Work @default.
- W1036150892 sameAs 1036150892 @default.
- W1036150892 citedByCount "7" @default.
- W1036150892 countsByYear W10361508922019 @default.
- W1036150892 countsByYear W10361508922021 @default.
- W1036150892 countsByYear W10361508922022 @default.
- W1036150892 crossrefType "book-chapter" @default.
- W1036150892 hasAuthorship W1036150892A5043815688 @default.
- W1036150892 hasAuthorship W1036150892A5081119595 @default.
- W1036150892 hasConcept C138885662 @default.
- W1036150892 hasConcept C139719470 @default.
- W1036150892 hasConcept C1491633281 @default.
- W1036150892 hasConcept C158525013 @default.
- W1036150892 hasConcept C162324750 @default.
- W1036150892 hasConcept C185592680 @default.
- W1036150892 hasConcept C2778348673 @default.
- W1036150892 hasConcept C41895202 @default.
- W1036150892 hasConcept C55493867 @default.
- W1036150892 hasConcept C92488253 @default.
- W1036150892 hasConceptScore W1036150892C138885662 @default.
- W1036150892 hasConceptScore W1036150892C139719470 @default.
- W1036150892 hasConceptScore W1036150892C1491633281 @default.
- W1036150892 hasConceptScore W1036150892C158525013 @default.
- W1036150892 hasConceptScore W1036150892C162324750 @default.
- W1036150892 hasConceptScore W1036150892C185592680 @default.
- W1036150892 hasConceptScore W1036150892C2778348673 @default.
- W1036150892 hasConceptScore W1036150892C41895202 @default.
- W1036150892 hasConceptScore W1036150892C55493867 @default.
- W1036150892 hasConceptScore W1036150892C92488253 @default.
- W1036150892 hasLocation W10361508921 @default.
- W1036150892 hasLocation W10361508922 @default.
- W1036150892 hasOpenAccess W1036150892 @default.
- W1036150892 hasPrimaryLocation W10361508921 @default.
- W1036150892 hasRelatedWork W1968006223 @default.
- W1036150892 hasRelatedWork W2065993041 @default.
- W1036150892 hasRelatedWork W2122781567 @default.
- W1036150892 hasRelatedWork W2278411437 @default.
- W1036150892 hasRelatedWork W2297542447 @default.
- W1036150892 hasRelatedWork W2314763380 @default.
- W1036150892 hasRelatedWork W2365328566 @default.
- W1036150892 hasRelatedWork W2386076595 @default.
- W1036150892 hasRelatedWork W2409335757 @default.
- W1036150892 hasRelatedWork W4236082445 @default.
- W1036150892 isParatext "false" @default.
- W1036150892 isRetracted "false" @default.
- W1036150892 magId "1036150892" @default.
- W1036150892 workType "book-chapter" @default.