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- W1036351112 abstract "为了探索转座子介导技术在鸡胚中的转染效果，本研究采用鸡蛋开窗法将Sleeping Beauty（SBl单质粒转座载体pT2-SV40-EGFP-SB11注射至孵化36h鸡（Gallus domesticus）胚盘中，同时设开窗不注射组和不开窗组。利用体视荧光显微镜和RT—PCR方法检测增强型绿色荧光蛋白基因（EGFP）表达率，并比较不同阶段的胚胎成活率、孵化率以及蛋重变化。结果表明，EGFP在鸡胚胎中获得较高的表达率f40％～89％）；开窗组和不开窗组在胚胎发育早期蛋重变化差异不显著（P〉0．05），而后期开窗注射组的蛋重比例显著低于不开窗组（P〈0．05）；三组胚胎的成活率和孵化率在整个发育过程中变化显著（P〈0．05）。本实验初步证明了经改良的鸡蛋开窗法可用于鸡胚转基因研究，并初步建立了SB转座子介导外源基因在鸡胚中转染方法。" @default.
- W1036351112 created "2016-06-24" @default.
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- W1036351112 date "2013-02-23" @default.
- W1036351112 modified "2023-09-28" @default.
- W1036351112 title "Sleeping Beauty（SB）转座子介导增强型绿色荧光蛋白基因（EGFP）体内转染鸡胚胎" @default.
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