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• W103635798 abstract "Event Abstract Back to Event Methamphetamine induces neuroinflammation and neuronal dysfunction in the mice hippocampus Joana Goncalves1, 2*, Sofia Baptista1, 3, Tania Martins1, 2, Nuno Milhazes4, Fernanda Borges4, Carlos Fontes-Ribeiro1, 2, Joao O. Malva3, 5 and Ana P. Silva1, 2 1 University of Coimbra, Institute of Pharmacology and Experimental Therapeutics, Faculty of Medicine, Portugal 2 University of Coimbra, Institute of Biomedical Research on Light and Image (IBILI), Faculty of Medicine, Portugal 3 University of Coimbra, Center for Neuroscience and Cell Biology, Portugal 4 University of Porto, Chemistry Department, Faculty of Pharmacy, Portugal 5 University of Coimbra, Institute of Biochemistry, Faculty of Medicine, Portugal It has been suggested that the activation of microglial cells and astrocytes, beyond neuronal dysfunction, may also contribute to methamphetamine (METH)-induced neuropathology. In the present study, we first demonstrate that a single acute METH administration (30 mg/kg; intraperitoneal injection), in male C57BL/6J wild-type mice, causes an inflammatory process in the hippocampus. This was characterized by astrocytes reactivity, microglia activation and alterations in the tumor necrosis factor (TNF) system. In fact, we observed an upregulation of glial fibrillary acidic protein (GFAP) levels at 1 h and 24 h post-METH injection and an increase in macrophage-1 (Mac-1; CD11b) immunoreactivity at 24 h and 3 days post-METH injection. Since METH induced a significant gliosis, and knowing that these cells are the main source of pro-inflammatory cytokines, we further evaluated possible changes in protein levels of TNF-α and their receptors. We verified that, post-METH treatment, both TNF-α and TNF receptor 1 (TNFR1) protein levels were upregulated at 7 days and 1 h and 24 h, respectively. However, we did not observe any changes in the TNF receptor 2 (TNFR2) protein levels. Furthermore, we investigated the effect of METH on hippocampal neurons and we observed a significant decrease in β-tubulin III (Tuj-1) protein levels following 24 h, 3 d and 7 d of METH treatment. Thus, to evaluate if all these changes observed after an acute administration of METH cause cell death, we analyzed the protein levels of caspase-3, but no alterations were observed. Moreover, we also examined the changes in the levels of several presynaptic and postsynaptic proteins, and our results corroborate the hypothesis of neuronal dysfunction. Indeed, syntaxin-1 protein levels were found to be decreased in the hippocampus following 3 days and 7 days of METH administration. In contrast, METH induced a significant increase in synaptophysin protein levels after 1 h and 24 h, but with no alterations in the levels of synaptosome-associated protein of 25000 daltons (SNAP25) and postsynaptic density 95 (PSD-95). In addition, we also observed that METH decreased calbindin D28k protein levels at the all time-points analyzed (1 h, 24 h, 3 days and 7 days), whereas tau levels only decreased 7 days post-administration. Taken together, our results demonstrate that a single acute administration of METH induces an inflammatory process and neuronal dysfunction.This work was supported by FCT, Portugal, Project PTDC/SAU-FCF/67053/2006 and Fellowship SFRH/BD/35893/2007 Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Neuronal Communication Citation: Goncalves J, Baptista S, Martins T, Milhazes N, Borges F, Fontes-Ribeiro C, Malva JO and Silva AP (2009). Methamphetamine induces neuroinflammation and neuronal dysfunction in the mice hippocampus. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.116 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Aug 2009; Published Online: 11 Aug 2009. * Correspondence: Joana Goncalves, University of Coimbra, Institute of Pharmacology and Experimental Therapeutics, Faculty of Medicine, Alicante, Portugal, jgoncalves@cnc.cj.uc.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Joana Goncalves Sofia Baptista Tania Martins Nuno Milhazes Fernanda Borges Carlos Fontes-Ribeiro Joao O Malva Ana P Silva Google Joana Goncalves Sofia Baptista Tania Martins Nuno Milhazes Fernanda Borges Carlos Fontes-Ribeiro Joao O Malva Ana P Silva Google Scholar Joana Goncalves Sofia Baptista Tania Martins Nuno Milhazes Fernanda Borges Carlos Fontes-Ribeiro Joao O Malva Ana P Silva PubMed Joana Goncalves Sofia Baptista Tania Martins Nuno Milhazes Fernanda Borges Carlos Fontes-Ribeiro Joao O Malva Ana P Silva Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W103635798 title "Methamphetamine induces neuroinflammation and neuronal dysfunction in the mice hippocampus" @default.
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