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- W1036509667 abstract "'Senga Sengana' strawberry shoots were stored in vitro at 4°C in the dark or at 23°C in the light for 24 months without subculture. Every 6 months, for sampled shoots, we determined the survival and multiplication ability in three subsequent subcultures, and the rooting ability after the second subculture. The standard storage medium did not contain cytokinin or auxin. It was modified by increasing the glucose or agar concentration, or by adding mannitol or the growth retardants uniconazol or paclobutrazol. The survival rate for both the shoots stored at 4°C in the dark for 24 months and for those stored at 23°C in the light for 6 months was 100%. The maximal storage period at 23°C was 12 months, which 100% or 76% of the shoots survived, depending on the experiment. The period of storage was the most important factor affecting shoot multiplication after storage under both sets of temperature and light conditions. The ability of cultures to produce axillary shoots decreased with the duration of storage, more in the first than in the second subculture, in comparison to the control cultures, which were subcultured every 5 weeks. The typical multiplication potential recovered in the third subculture. The applied storage conditions did not affect the rooting ability of shoots after the second subculture (100% of shoots rooted), or the quality and alignment of plants after acclimatization. Increasing the concentration of glucose or agar or adding mannitol to the storage medium (i.e. compounds decreasing the availability of water), or adding growth retardants did not affect the survival of shoots stored at 4°C. A higher concentration of glucose or agar, or supplementary mannitol further increased the multiplication ability of shoots stored at 4°C for 6 months. Using CultuSac® polyethylene bags further improved the multiplication of cultures stored at 23°C for 6 months." @default.
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- W1036509667 date "2008-01-01" @default.
- W1036509667 modified "2023-09-24" @default.
- W1036509667 title "THE POST-EFFECT OF IN VITRO STORAGE ON SHOOT MULTIPLICATION AND ROOTING OF 'SENGA SENGANA' STRAWBERRY SHOOTS" @default.
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