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- W1036623587 abstract "To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21^WAF-1/CIP1 gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-vi-ral vector GET gene delivery system was constructed. The malignant human glioma cell llne U251MG was transfected in vitro with β-galactosidase gene( reporter gene) and p21^WAF-1/CIP1 gene (therapeutic gene) using the GET system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21^WAF-1/CIP1 gene in transfected U251MG cell was examined by immunohistochemitry staining. Results: The highest transfer rate of exogenous gene was 70%. After transfection with p21^WAF-l/CIP1 gene,the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently.FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21^WAF-l/CIP1 gene can induce apoptosis of glio-ma cell and inhibit its growth." @default.
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- W1036623587 date "2003-01-01" @default.
- W1036623587 modified "2023-09-22" @default.
- W1036623587 title "In vitro effect of p21^WAF-1／CIPI gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system" @default.
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