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- W1036628968 abstract "A range of methods has been developed for the assay of polysaccharide endohydrolases, and these include viscosimetric and nephelometric methods and procedures based on the measurement of increase in reducing sugar equivalents and on the rate of release of soluble, dye-labeled fragments on hydrolysis of chromogenic polysaccharide substrates. Assays based on the use of chromogenic (dye-labeled) substrates have several advantages over more conventional assays including specificity and simplicity in use. However, as dyeing generally reduces the solubility of the polysaccharide, most commercially available dye-labeled substrates are insoluble and have the inherent disadvantages of heterogeneity in the assay tube and the difficulties associated with dispensing a solid substrate routinely with accuracy. This chapter describes methods for the preparation of soluble dye-labeled substrates for the assay β-D-mannanase, endo-l,4-β-D-glucanase , endo-l,3(4)-β-o-glucanase, and α-amylase." @default.
- W1036628968 created "2016-06-24" @default.
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- W1036628968 date "1988-01-01" @default.
- W1036628968 modified "2023-10-16" @default.
- W1036628968 title "Soluble, dye-labeled polysaccharides for the assay of endohydrolases" @default.
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- W1036628968 doi "https://doi.org/10.1016/0076-6879(88)60108-x" @default.
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