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- W1036672321 abstract "Publisher Summary This chapter discusses the synthesis of mannan synthetase from yeast. A particulate preparation from disrupted yeast protoplasts catalyzes the incorporation of mannose from galactokinase diphosphate (GDP)-l4C-mannose into a mannose polysaccharide that precipitates with a copper reagent, as does authentic yeast mannan. Two assay methods are described in the chapter. In the first, the reaction mixture is submitted to paper electrophoresis; the radioactive mannan formed remains at the starting line, while the substrate and decomposition products travel toward the anode. In the other, more specific but also much more laborious method, the radioactive polysaccharide is isolated before counting. A blank, prepared by adding GDP-mannose to the reaction mixture just before transfer to the paper, is counted in the same way and subtracted. The alcohol precipitate contains glycogen and other substances besides mannan. The reagents used, procedure followed, and the enzyme preparation is also described in the chapter. All operations are carried out at 0–2°, unless otherwise stated." @default.
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- W1036672321 date "1966-01-01" @default.
- W1036672321 modified "2023-10-18" @default.
- W1036672321 title "[71] Mannan synthetase from yeast" @default.
- W1036672321 cites W2070319037 @default.
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- W1036672321 doi "https://doi.org/10.1016/0076-6879(66)08075-3" @default.
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