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- W1036706853 abstract "This chapter discusses the preparation and assay of chloroplast coupling factor (CF1). Treatment of chloroplasts with a dilute EDTA solution liberates a soluble coupling factor. The active factor in the EDTA extract is identical to coupling factor CF1, which contains a latent Ca2+ dependent ATPase. An assay for CF1 with EDTA-treated chloroplasts or with subchloroplast particles obtained by sonication of chloroplasts in the presence of phospholipids (CP particles) is described in this chapter. Because EDTA-treated chloroplasts cannot be stored and the degree of resolution of CP particles is somewhat variable, an assay with stable subchloroplast particles, which are completely resolved with respect to CF1, is used. In addition to coupling activity, CF1 has also a latent Ca2+-dependent ATPase activity, which can be activated by heat treatment, trypsin digestion, or prolonged incubation at high concentrations of dithiothreitol. The ATPase activity provides a rapid and convenient assay of the enzyme and is especially useful in the course of purification. The activity of the enzyme is assayed by determining the amount of Pi liberated by the enzymatic hydrolysis of ATP." @default.
- W1036706853 created "2016-06-24" @default.
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- W1036706853 date "1971-01-01" @default.
- W1036706853 modified "2023-10-18" @default.
- W1036706853 title "[49] Preparation and assay of chloroplast coupling factor CF1" @default.
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- W1036706853 doi "https://doi.org/10.1016/s0076-6879(71)23125-6" @default.
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