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- W1036715301 abstract "The use of cell cultures in the screening for variant cell types and for the isolation of potentially useful phenotypes in crop plants has been emphasized (e.g. [5]), and selection for drug and, in particular, herbicide resistance at the cell level and subsequent regeneration of resistant plants have met with certain success (see [14, 17]). The biochemical characterization of resistant cell lines, and the genetic characterization of the regenerated mutant plants may allow the identification of the site of action of a herbicide, a striking example being the recent demonstration that the target of sulfonylurea herbicides in higher plants is the first enzyme specific for the biosynthesis of the branched chain amino acids, acetolactate synthase [6, 7]. Resistance to a herbicide may, however, be acquired by a number of mechanisms, not all of which need be related with the primary site of action of the particular agent. For example, reduced uptake and translocation, or increased detoxification may determine the differential intraspecific responses to herbicides [16]. Target-related mechanisms of resistance to drugs and other metabolic inhibitors can involve either a reduced affinity of the target site for binding the inhibitor, or an increased production of target sites. The latter phenomenon has been intensely studied in bacteria and, in particular, in cultured animal cells, in which specific genes can undergo amplification when the cells are placed under selective conditions (see [22, 26])." @default.
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- W1036715301 date "1985-01-01" @default.
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- W1036715301 title "A Glyphosate-Tolerant Plant Tissue Culture" @default.
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- W1036715301 doi "https://doi.org/10.1007/978-3-642-70717-9_34" @default.
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