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- W103678509 abstract "We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into antisense RNA probes by in vitro transcription. The quality of each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay. RNA probes are hybridized to fixed, mixed-staged Drosophila embryos in 96-well plates. The resulting stained embryos can be examined and photographed immediately or stored at 4oC for later analysis. Starting with fixed, staged embryos, the protocol takes 6 d from probe template production through hybridization. Preparation of fixed embryos requires a minimum of 2 weeks to collect embryos representing all stages. The method has been used to determine the expression patterns of over 6,000 genes throughout embryogenesis." @default.
- W103678509 created "2016-06-24" @default.
- W103678509 creator A5073571418 @default.
- W103678509 date "2010-03-16" @default.
- W103678509 modified "2023-09-24" @default.
- W103678509 title "Determination of gene expression patterns using high-throughput RNA in situ hybridizaion to whole-mount Drosophila embryos" @default.
- W103678509 hasPublicationYear "2010" @default.
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