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- W103679180 abstract "SummaryThe treatment of tissue sections to enhance probe access to target mRNA is a critical step in the methodology of in situ hybridization. We have overcome some of the problems encountered in enzyme-based treatment of tissue sections by the application of microwave oven heating. Microwave treatment can (1) replace proteinase K digestion for frozen sections; (2) enhance proteinase K digestion in paraffin sections; (3) denature mRNA structure to enable better probe access; (4) preserve tissue architecture; and (5) inactivate endogenous alkaline phosphatase within tissue sections to reduce background with immunohistochemistry-based probe detection." @default.
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- W103679180 date "2006-06-30" @default.
- W103679180 modified "2023-10-18" @default.
- W103679180 title "Treatment of Tissue Sections for <i>In Situ</i> Hybridization" @default.
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- W103679180 doi "https://doi.org/10.1385/1-59745-007-3:1" @default.
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