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- W1036823062 abstract "Publisher Summary This chapter focuses on some of the possibilities and future potentials inherent in fluorescence methods as well as to some of the difficulties in making absolute measurements and obtaining corrected spectra. Fluorescence involves the absorption of energy by a molecule with a transition from the ground state to one of several excited states followed by rapid internal conversion to the lowest energy excited state and finally by a return to the ground state with the emission of light. Fluorescence measurements are usually more selective than spectrophotometry. The chapter also describes the instrumental aspects of fluorescence, a typical fluorometer, and detailed information about the components utilized in building such an instrument. The specific components are light sources, igniters, light-source housings, monochromators, and detectors. A number of commercial fluorometers and spectrophotofluorometers are also discussed. The potential procedure for obtaining corrected spectra involves the use of digital computing facilities. Although some of the instruments give partially corrected spectra, it is important to understand the errors involved in fluorescence measurements and the type of corrections that must be applied in most cases." @default.
- W1036823062 created "2016-06-24" @default.
- W1036823062 creator A5006148171 @default.
- W1036823062 creator A5054688355 @default.
- W1036823062 date "1967-01-01" @default.
- W1036823062 modified "2023-10-11" @default.
- W1036823062 title "[87] Fluorescence measurements" @default.
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- W1036823062 doi "https://doi.org/10.1016/s0076-6879(67)11091-4" @default.
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