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- W1036842977 abstract "The primary regulator of the reproductive cycle is the hypothalamic decapeptide gonadotropin-releasing hormone (GnRH), which stimulates gonadotropin release from the anterior pituitary. Immobilized forms of avidin can be used for the isolation of receptors. This can be accomplished either by binding the biotinylated hormone to avidin columns followed by subsequent interaction with the solubilized receptor or by prior incubation of the biotinylated hormone with the solubilized receptor and then immobilization on avidin columns. A thorough characterization of the biotinylated peptide hormone is necessary if structure–function studies are to be performed. Because peptide hormones are often not available in large quantities and fractionation of active biotinylated species may reduce the available amounts of peptide, it can be a considerable advantage to use the microsequencing techniques for determination of biotin location and the purity of individual fractions. Small peptides can be sequenced directly and larger proteins can be characterized after fragmentation by chemical or enzymatic means." @default.
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- W1036842977 date "1990-01-01" @default.
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- W1036842977 title "[29] Purification of gonadotropin-releasing hormone receptors" @default.
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- W1036842977 doi "https://doi.org/10.1016/0076-6879(90)84283-m" @default.
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