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- W103685494 abstract "AbstractContinuous Magnetic Extraction (CME) is a process for the separation and purification of proteins from crude feedstocks. Magnetic particles with functional surface groups, e.g., ion exchange ligands, serve as carriers for the target protein. Together with a mixture of thermoresponsive surfactants and binding buffer these carriers are added to the feed containing the target protein. After binding the target within minutes, the mixture is heated above the LCST of the surfactant and phase separation is induced. Through selective partitioning of the carrier particles to the top phase and partitioning of contaminants to the bottom phase of the system, purification is possible. The proof-of-concept of this bioseparation process has been recently released, and new devices for CME have been developed within the framework of the EU project MagPro2Life and the respective results are reported in this chapter. Several particle types and feed streams, representing a broad field of potential applications, were tested with regard to their suitability for the CME process.KeywordsLower Critical Solution TemperatureFeed StreamBottom PhaseSurfactant Rich PhaseSeparation ChamberThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W103685494 created "2016-06-24" @default.
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- W103685494 date "2014-01-01" @default.
- W103685494 modified "2023-09-23" @default.
- W103685494 title "Continuous Magnetic Extraction for Protein Purification" @default.
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- W103685494 doi "https://doi.org/10.1007/978-3-662-43899-2_10" @default.
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