FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1036896149> ?p ?o ?g. }

• W1036896149 endingPage "96" @default.
• W1036896149 startingPage "91" @default.
• W1036896149 abstract "△9-tetrahydrocannabinol, the major psychoactive cannabinoid of marijuana, modulates immune cells in vivo and in vitro. It is possible that the drug exerts it’s effect either by inserting into and disrupting the cell membrane (nonreceptor mechanism) or by binding to a cannabinoid receptor moiety and thus altering cell function through some form of signal transduction. In the present study, we confirm and extend the findings that mouse and human immune cells express specific cannabinoid binding sites and cannabinoid receptor mRNA. Reverse transcription-polymerase chain reaction analysis showed the presence of receptor mRNA not only in the neuroblastoma cell line (N18TG-2), but also in mouse splenocytes and in cell lines such as NKB61A2 (a mouse natural killer-like), CTLL2 (a mouse IL2-dependent T cell), THP-1 (a human monocytic cell) and Raji (a human B cell) but not in Jurkat (a human T cell). Furthermore, the receptor mRNA was expressed in purified populations of resting splenic T and B lymphocytes but not in resting populations of enriched splenic macrophages. Finally, LPS-stimulated Raji and PMA-stimulated THP-1 human cell lines showed increased levels of the cannabinoid receptor mRNA. These results suggest cannabinoid receptors have biological relevance in lymphoid cells because: receptor mRNA is detected in some resting immune cells but not others and the mRNA increases during cell activation.The major psychoactive component of marijuana, A9-tetrahydrocannabinol (THC), has been shown to modulate human and mouse immune responses both in vitro and in vivo (1,2). A few reports have examined the molecular basis for this drug-induced modulation and suggest that THC suppresses intracellular calcium mobilization during lymphocyte activation (3), modulation of membrane fatty acids in macrophages (4) and lymphocytes (5), suppression of functional IL2 receptors (6), and modulation of cytokine release from macrophages (7).A putative cannabinoid receptor has been identified in neuronal tissues from a variety of mammalian species by in situ hybridization and binding studies (8-10). Recently, a cannabinoid receptor gene was cloned from a rat brain cDNA library and identified as a G protein-coupled receptor (11). Expression of the receptor has been reported in nonneuronal tissues such as the testis (12) and, recently, in unfractionated mouse spleen cells (13) and human leukocytes (14) but it’s function in these cells is unknown. These findings prompted us to examine in greater detail the distribution and regulation of the brain receptor in various immune cell populations. The results show receptor message is detected in some subpopulations but not in others and is increased during cell line activation. These results are consistent with a biological function of the receptor in immune cells. The relationship of this putative function, however, to THC-induced immunomodulation is currently not known." @default.
• W1036896149 created "2016-06-24" @default.
• W1036896149 creator A5028159080 @default.
• W1036896149 creator A5032965517 @default.
• W1036896149 creator A5072207657 @default.
• W1036896149 date "1995-01-01" @default.
• W1036896149 modified "2023-10-14" @default.
• W1036896149 title "Expression of Cannabinoid Receptor mRNA in Murine and Human Leukocytes" @default.
• W1036896149 cites W1685180105 @default.
• W1036896149 cites W1989073099 @default.
• W1036896149 cites W2011131010 @default.
• W1036896149 cites W2011287768 @default.
• W1036896149 cites W2013220024 @default.
• W1036896149 cites W2026687408 @default.
• W1036896149 cites W2027150919 @default.
• W1036896149 cites W2029181309 @default.
• W1036896149 cites W2041580452 @default.
• W1036896149 cites W2066534120 @default.
• W1036896149 cites W2075894678 @default.
• W1036896149 cites W2140508950 @default.
• W1036896149 doi "https://doi.org/10.1007/978-1-4615-1951-5_13" @default.
• W1036896149 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7545349" @default.
• W1036896149 hasPublicationYear "1995" @default.
• W1036896149 type Work @default.
• W1036896149 sameAs 1036896149 @default.
• W1036896149 citedByCount "21" @default.
• W1036896149 countsByYear W10368961492013 @default.
• W1036896149 countsByYear W10368961492016 @default.
• W1036896149 crossrefType "book-chapter" @default.
• W1036896149 hasAuthorship W1036896149A5028159080 @default.
• W1036896149 hasAuthorship W1036896149A5032965517 @default.
• W1036896149 hasAuthorship W1036896149A5072207657 @default.
• W1036896149 hasConcept C148001335 @default.
• W1036896149 hasConcept C153911025 @default.
• W1036896149 hasConcept C158026453 @default.
• W1036896149 hasConcept C170493617 @default.
• W1036896149 hasConcept C179464577 @default.
• W1036896149 hasConcept C185592680 @default.
• W1036896149 hasConcept C203014093 @default.
• W1036896149 hasConcept C2776090121 @default.
• W1036896149 hasConcept C2776885963 @default.
• W1036896149 hasConcept C2780871563 @default.
• W1036896149 hasConcept C54355233 @default.
• W1036896149 hasConcept C55493867 @default.
• W1036896149 hasConcept C81885089 @default.
• W1036896149 hasConcept C86803240 @default.
• W1036896149 hasConcept C8891405 @default.
• W1036896149 hasConcept C93248783 @default.
• W1036896149 hasConcept C95444343 @default.
• W1036896149 hasConceptScore W1036896149C148001335 @default.
• W1036896149 hasConceptScore W1036896149C153911025 @default.
• W1036896149 hasConceptScore W1036896149C158026453 @default.
• W1036896149 hasConceptScore W1036896149C170493617 @default.
• W1036896149 hasConceptScore W1036896149C179464577 @default.
• W1036896149 hasConceptScore W1036896149C185592680 @default.
• W1036896149 hasConceptScore W1036896149C203014093 @default.
• W1036896149 hasConceptScore W1036896149C2776090121 @default.
• W1036896149 hasConceptScore W1036896149C2776885963 @default.
• W1036896149 hasConceptScore W1036896149C2780871563 @default.
• W1036896149 hasConceptScore W1036896149C54355233 @default.
• W1036896149 hasConceptScore W1036896149C55493867 @default.
• W1036896149 hasConceptScore W1036896149C81885089 @default.
• W1036896149 hasConceptScore W1036896149C86803240 @default.
• W1036896149 hasConceptScore W1036896149C8891405 @default.
• W1036896149 hasConceptScore W1036896149C93248783 @default.
• W1036896149 hasConceptScore W1036896149C95444343 @default.
• W1036896149 hasLocation W10368961491 @default.
• W1036896149 hasLocation W10368961492 @default.
• W1036896149 hasOpenAccess W1036896149 @default.
• W1036896149 hasPrimaryLocation W10368961491 @default.
• W1036896149 hasRelatedWork W1819429514 @default.
• W1036896149 hasRelatedWork W1966146877 @default.
• W1036896149 hasRelatedWork W1999203299 @default.
• W1036896149 hasRelatedWork W2002785222 @default.
• W1036896149 hasRelatedWork W2015614945 @default.
• W1036896149 hasRelatedWork W2038107480 @default.
• W1036896149 hasRelatedWork W2070592640 @default.
• W1036896149 hasRelatedWork W2077182496 @default.
• W1036896149 hasRelatedWork W2140269042 @default.
• W1036896149 hasRelatedWork W2952752134 @default.
• W1036896149 isParatext "false" @default.
• W1036896149 isRetracted "false" @default.
• W1036896149 magId "1036896149" @default.
• W1036896149 workType "book-chapter" @default.