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- W1037139618 abstract "Publisher Summary This chapter discusses the determination of quinones from algae and higher plants. In the case of determination of quinones from higher plants, spinach leaves in 200-g quantities are ground in 1.5 liters of grinding medium for 1 minute at low speed (15,500 rpm without a load). The chlorophyll content of spinach chloroplasts is determined by Arnon's method. The green supernatant is used for a spectrophotometric assay for chlorophyll a (663 nm) and b (645 nm) read against an acetone blank. The extract from chloroplasts or whole leaves containing plastoquinones and other quinones is transferred to a separatory funnel. After separation of the initial two phases, the green lipid epiphase is set aside while the aqueous hypophase is rewashed with 500 ml of heptane, which is later added to the previously collected heptane epiphase. Plastoquinones of the A, B, and C types, which occur in all taxonomic divisions of higher plants have also been found in all classes of algae, including representatives of greens, yellow-greens, blue-greens, reds, browns, and the flagellate, Euglena. Quinones from algae can be extracted by the heptane–isopropanol–water (1:1:1) method, or by the alternative quinone extraction procedures with acetone." @default.
- W1037139618 created "2016-06-24" @default.
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- W1037139618 date "1971-01-01" @default.
- W1037139618 modified "2023-09-25" @default.
- W1037139618 title "[37] Quinones in algae and higher plants" @default.
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- W1037139618 doi "https://doi.org/10.1016/s0076-6879(71)23113-x" @default.
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