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- W1037388637 abstract "Publisher Summary This chapter discusses the high-efficiency entrapment of enzymes in resealed red cell ghosts by dialysis. The utility of erythrocytes as drug carders was increased dramatically when the original dilution procedures for erythrocyte lysis and drug entrapment were replaced by high-hematocrit dialysis procedures. These dialysis methods substantially improved the incorporation etticiencies for the rare and valuable biochemicals, which investigators wanted to entrap in erythrocytes. Several different procedures for dialysis loading of erythrocytes have been reported, but all take advantage of the same principle that the semipermeable dialysis membrane maximizes the intracellular: extracellular volume ratio for macromolecules during lysis and resealing, yet also allows a free flow of small ions, which are required for lysis and resealing of the erythrocytes. It is this intracellular: extracellular volume ratio during the time that the erythrocyte membrane is permeable that determines the incorporation yield for entrapment. This chapter discusses dialysis tubing. The dialysis tubing may be treated with chelating agents to remove possible heavy metal contaminants or autoclaved to obtain sterility without affecting its performance." @default.
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- W1037388637 date "1987-01-01" @default.
- W1037388637 modified "2023-09-24" @default.
- W1037388637 title "[22] High-efficiency entrapment of enzymes in resealed red cell ghosts by dialysis" @default.
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- W1037388637 doi "https://doi.org/10.1016/0076-6879(87)49060-5" @default.
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