FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1039968132> ?p ?o ?g. }

• W1039968132 abstract "The grape variety Sauvignon Blanc (SB) is the flagship of New Zealand???s wine industryand accounted for over 75 % of the value of total wine exports in 2008. Two volatilethiols, 3-mercaptohexan-1-ol (3MH) and 3-mercaptohexyl-acetate (3MHA), reminiscent of grapefruit and passion fruit respectively, are critical for the main varietal characters in New Zealand SB. These aromatic thiols are not present in the grape juice, but are synthesized and released by the yeast during alcoholic fermentation from non-aromatic precursors. The aim of this work was to elucidate the underlying genetics of volatile thiol synthesisin yeast (Saccharomyces cerevisiae) during alcoholic fermentation of grape juice. A gene-deletion strategy was chosen for the investigation of putative genes influencing 3MH and 3MHA release.The first part of this thesis optimized fermentation conditions in grape-juice-based media, which enabled auxotrophic laboratory strains, derived from S288C, to ferment grapejuice to completion with high efficiency. Key steps to achieving this goal were the supplementation of the grape juice with higher than recommended amounts of amino acids, which increased the fermentation rate of auxotrophic yeast strains. Lysine auxotrophic strains especially benefited from this measure. In combination with the dilution of SB grape juice by 25 % with synthetic grape juice without sugars, the auxotrophic laboratory yeast BY4743 was able to metabolize all sugars in the grape juice-based media in a time frame similar to that of a commercial wine yeast. The key properties of the resulting wine were comparable to wine made with a commercial wine yeast under the same conditions.In the second part, these newly developed fermentation conditions were employed toscreen 69 single-gene deletion strains in the laboratory yeast background BY4743. Thelist of the 69 candidate genes was compiled by combining existing knowledge about thiolproduction in yeast with the mining of several biological databases. Screening of the single-gene deletions revealed 17 genes which caused biologically relevant increases or decreases in volatile thiol production, but none abolished it. The majority of the 17 genes were related to the sulfur and nitrogen metabolism in yeast. A subset of these thiol-influencing genes were also deleted in a wine yeast, and were overexpressed in both wine yeast and laboratory yeast, to gain more insight in their regulatory effects. The findings confirmed that sulfur and nitrogen metabolism in yeast were important in regulating 3MH and 3MHA synthesis. Different sulfur and nitrogen sources were added to the grape must prior to fermentation and their effect on thiol release was studied. It was found that nitrogen sources urea and DAP, as well as, the sulfur compound S-ethyl-L-cysteine (SEC) increased 3MH and 3MHA concentrations in the resulting wines. The addition of cysteine to grape juice fermented with wine yeast deleted in genes CYS3 and CYS4 more than doubled total thiol production. Mapping approaches to investigate…" @default.
• W1039968132 created "2016-06-24" @default.
• W1039968132 creator A5009813658 @default.
• W1039968132 date "2009-01-01" @default.
• W1039968132 modified "2023-09-28" @default.
• W1039968132 title "Identification of yeast genes involved in sauvignon blanc aroma development" @default.
• W1039968132 hasPublicationYear "2009" @default.
• W1039968132 type Work @default.
• W1039968132 sameAs 1039968132 @default.
• W1039968132 citedByCount "2" @default.
• W1039968132 countsByYear W10399681322012 @default.
• W1039968132 countsByYear W10399681322013 @default.
• W1039968132 crossrefType "dissertation" @default.
• W1039968132 hasAuthorship W1039968132A5009813658 @default.
• W1039968132 hasConcept C100544194 @default.
• W1039968132 hasConcept C185592680 @default.
• W1039968132 hasConcept C2775920511 @default.
• W1039968132 hasConcept C2777576037 @default.
• W1039968132 hasConcept C2779222958 @default.
• W1039968132 hasConcept C2779737534 @default.
• W1039968132 hasConcept C2780563676 @default.
• W1039968132 hasConcept C3018968983 @default.
• W1039968132 hasConcept C31903555 @default.
• W1039968132 hasConcept C36442339 @default.
• W1039968132 hasConcept C523546767 @default.
• W1039968132 hasConcept C54355233 @default.
• W1039968132 hasConcept C55493867 @default.
• W1039968132 hasConcept C55952523 @default.
• W1039968132 hasConcept C59920403 @default.
• W1039968132 hasConcept C78945660 @default.
• W1039968132 hasConcept C86803240 @default.
• W1039968132 hasConcept C98861065 @default.
• W1039968132 hasConceptScore W1039968132C100544194 @default.
• W1039968132 hasConceptScore W1039968132C185592680 @default.
• W1039968132 hasConceptScore W1039968132C2775920511 @default.
• W1039968132 hasConceptScore W1039968132C2777576037 @default.
• W1039968132 hasConceptScore W1039968132C2779222958 @default.
• W1039968132 hasConceptScore W1039968132C2779737534 @default.
• W1039968132 hasConceptScore W1039968132C2780563676 @default.
• W1039968132 hasConceptScore W1039968132C3018968983 @default.
• W1039968132 hasConceptScore W1039968132C31903555 @default.
• W1039968132 hasConceptScore W1039968132C36442339 @default.
• W1039968132 hasConceptScore W1039968132C523546767 @default.
• W1039968132 hasConceptScore W1039968132C54355233 @default.
• W1039968132 hasConceptScore W1039968132C55493867 @default.
• W1039968132 hasConceptScore W1039968132C55952523 @default.
• W1039968132 hasConceptScore W1039968132C59920403 @default.
• W1039968132 hasConceptScore W1039968132C78945660 @default.
• W1039968132 hasConceptScore W1039968132C86803240 @default.
• W1039968132 hasConceptScore W1039968132C98861065 @default.
• W1039968132 hasLocation W10399681321 @default.
• W1039968132 hasOpenAccess W1039968132 @default.
• W1039968132 hasPrimaryLocation W10399681321 @default.
• W1039968132 hasRelatedWork W1984879872 @default.
• W1039968132 hasRelatedWork W1985016830 @default.
• W1039968132 hasRelatedWork W1993659312 @default.
• W1039968132 hasRelatedWork W1994163376 @default.
• W1039968132 hasRelatedWork W2023835998 @default.
• W1039968132 hasRelatedWork W2046242458 @default.
• W1039968132 hasRelatedWork W2062004100 @default.
• W1039968132 hasRelatedWork W2081307242 @default.
• W1039968132 hasRelatedWork W2083502716 @default.
• W1039968132 hasRelatedWork W2162746297 @default.
• W1039968132 hasRelatedWork W2296357968 @default.
• W1039968132 hasRelatedWork W2335856836 @default.
• W1039968132 hasRelatedWork W2464692717 @default.
• W1039968132 hasRelatedWork W2761287636 @default.
• W1039968132 hasRelatedWork W2886804869 @default.
• W1039968132 hasRelatedWork W2889569991 @default.
• W1039968132 hasRelatedWork W2908109932 @default.
• W1039968132 hasRelatedWork W3178549630 @default.
• W1039968132 hasRelatedWork W3186731157 @default.
• W1039968132 hasRelatedWork W3199336483 @default.
• W1039968132 isParatext "false" @default.
• W1039968132 isRetracted "false" @default.
• W1039968132 magId "1039968132" @default.
• W1039968132 workType "dissertation" @default.