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- W1040476052 abstract "Publisher Summary This chapter discusses the assay method, purification procedure, and properties of L-sorbose-1-phosphate reductase. L-sorbose-1-phosphate reductase functions in L-sorbose catabolism in wild-type Klebsiella pneumoniae , wherein it is induced by L-sorbose. It also functions as an essential enzyme in D-fructose catabolism in a genetically reconstructed strain of this organism, wherein it is constitutive and catalyzes the second reaction of the pathway: D-fructose → D-fructose 1-phosphate → D-mannitol 6-phosphate → D-fructose 6-phosphate. The rate of D-fructose 1-phosphate-dependent nicotinamide adenine dinucleotide dehydrogenase (NADH) oxidation is determined by measuring the rate of absorbance decrease at 340 nm. One unit of L-sorbose-1-phosphate reductase is defined as the amount that catalyzes the oxidation of 1 tzmol of NADH per minute in the standard assay. The enzyme exhibits no activity unless an activating divalent metal ion is included in the assay mixture. The steps involved in the purification procedure of the enzyme are (1) the preparation of cell extracts, (2) diethylaminoethyl (DEAE)-cellulose chromatography I and II, and (3) affinity chromatography I and II." @default.
- W1040476052 created "2016-06-24" @default.
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- W1040476052 date "1982-01-01" @default.
- W1040476052 modified "2023-09-27" @default.
- W1040476052 title "[42] l-sorbose-1-phosphate reductase" @default.
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- W1040476052 doi "https://doi.org/10.1016/s0076-6879(82)89044-7" @default.
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