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- W1040609877 abstract "目的对硅转化细胞基因组DNA异常甲基化进行研究,探讨硅的表遗传致癌机制.方法从结晶型硅(Si)转化BALB/c-3T3细胞中提取基因组DNA,经Mse1(甲基化非敏感性酶)单独消化或Mse1和BstU1(甲基化敏感性酶)联合消化,消化产物用甲基化敏感性内切酶指纹法(MSRF)进行分析,差异显示出异常甲基化基因片段,进一步将异常甲基化DNA片段亚克隆和序列测定,再与基因文库中的基因进行类比分析.结果发现硅转化细胞存在6条异常甲基化DNA(其中1条为高甲基化,5条有低甲基化现象),序列测定显示这些异常甲基化基因片段似乎来源于一些RNA转录和蛋白质翻译等基因家族.结论 DNA异常甲基化会导致基因表达激活或抑制,因此硅转化细胞基因组某些功能基因DNA异常甲基化导致的异常表达,可能间接是硅诱导细胞转化及其致癌作用的一种表遗传机制." @default.
- W1040609877 created "2016-06-24" @default.
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- W1040609877 date "2003-01-01" @default.
- W1040609877 modified "2023-09-23" @default.
- W1040609877 title "硅转化BALB／c-3T3细胞基因组DNA异常甲基化的研究" @default.
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