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- W1041445848 abstract "This chapter discusses the determination of lipoyl dehydrogenase from pig heart. Three suitable assays described are: reduced diphosphopyridine nucleotide (DPNH)–lipoyl reductase activity, DPNH–dichlorophenolindophenol reductase activity, and DPNH–ferricyanide reductase activity. In the case of DPNH–lipoyl reductase activity, the activity measured is dependent both on ionic strength and on the presence of DPN. Activity is measured spectrophotometrically at 340 mμ in the following reaction mixture at 25°; 2.5 ml of citrate buffer; 0.1 ml of bovine serum albumin in ethylenediaminetetraacetic acid (EDTA); 0.1 ml of DL-lipoic acid; 0.03 ml of DPNH, 0.03 ml of DPN; water and finally enzyme to a total volume of 3.0 ml. In the case of DPNH–dichlorophenolindophenol reductase activity, activity is measured spectrophotometrically at 600 mμ at 25° by following the reduction of the dye. Each cuvette contains 0.5 ml of phosphate buffer; 0.1 ml of bovine serum albumin in EDTA; 0.12 ml of dichlorophenolindophenol; 0.06 ml of DPNH; water and finally enzyme to a total volume of 3.0 ml. With the native enzyme this activity is small (0.4–0.5 micromole DPNH oxidized per minute per milligram protein). In the case of DPNH–ferricyanide reductase activity, activity is measured spectrophotometrically at 420 mμ at 25° by following the reduction of K 3 Fe(CN) 6 ." @default.
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- W1041445848 date "1966-01-01" @default.
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- W1041445848 title "[52] Lipoyl dehydrogenase from pig heart" @default.
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- W1041445848 doi "https://doi.org/10.1016/0076-6879(66)09059-1" @default.
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